Privalova L I, Katsnel'son B A, Osipenko A V
Biull Eksp Biol Med. 1977 Mar;83(3):342-5.
Peritoneal macrophages (PM) of Wistar rats harvested after the intraperitoneal injection of paraffin oil were destroyed by repeated freezing-thawing. When injected intratracheally to control rats or to those after 4 daily exposured to TiO2 dust, these macrophage destruction products (MDP) caused a significant rise of both the alveolar macrophages (AM) and the neutrophilic leukocytes (NL) counts in the pulmonary washing-outs; the mean NL/AM ratio increased several times as compared to rats injected with normal saline intratracheally. Thus, the response to the inert dust particles plus the exogenous MDP became similar to the one observed after the cytotoxic (for instance silica) particles inhalation. Enhancing the NL contribution to the inhaled particles phagocytosis, the MDP led to a significant decrease of the mean "Dust load" of a single AM, although the total number of the engulfed particles increased. The predominant attraction of granulocytes and particularly of the NL as compared to the peritoneal macrophages was also found in the peritoneal exudates of rats injected with the MDP or silica suspension intraperitoneally, while the alveolar phagocytosis was not influenced. In vitro the MDP was shown to stimulate the NL migration and to facilitate the O2 consumption by PM. A possible role of the MDP as a multipotent controlling factor of phagocytosis response is briefly discussed.
经腹腔注射石蜡油后收获的Wistar大鼠腹腔巨噬细胞（PM），通过反复冻融将其破坏。当将这些巨噬细胞破坏产物（MDP）经气管内注射给对照大鼠或每日暴露于TiO₂粉尘4天后的大鼠时，肺灌洗中的肺泡巨噬细胞（AM）和中性粒细胞（NL）计数均显著升高；与经气管内注射生理盐水的大鼠相比，平均NL/AM比值增加了几倍。因此，对惰性尘埃颗粒加外源性MDP的反应变得类似于吸入细胞毒性（如二氧化硅）颗粒后观察到的反应。MDP增强了NL对吸入颗粒吞噬作用的贡献，导致单个AM的平均“尘埃负荷”显著降低，尽管吞噬颗粒的总数增加。在经腹腔注射MDP或二氧化硅悬液的大鼠的腹腔渗出物中，也发现与腹腔巨噬细胞相比，粒细胞尤其是NL具有主要吸引力，而肺泡吞噬作用未受影响。在体外，MDP被证明可刺激NL迁移并促进PM的氧气消耗。简要讨论了MDP作为吞噬作用反应的多能控制因子的可能作用。
