Babikova R A, Bliumkin V N, Shal'nev B I, Polonskaia L B
Biull Eksp Biol Med. 1977 Mar;83(3):350-3.
The authors present morphological characteristics of primary monolayer cultures prepared from the pancreas of bovine fetuses. Combined treatment with trypsin and collalytine solutions (a preparation with collagenase activity) was used for dispersion of the tissue of the pancreas. Numerous epithelial cells corresponding by morphofunctional characteristics to beta-cells of the islets of Langerhans were contained in be cultures obtained; an aldehyde-fuchsin-positive granularity was revealed in the cytoplasm of these cells. Degranulation of these cells occurred under the effect of an increased glucose concentration in the nutrient medium.
作者展示了从牛胎儿胰腺制备的原代单层培养物的形态学特征。使用胰蛋白酶和胶原酶溶液（一种具有胶原酶活性的制剂）联合处理来分散胰腺组织。在所获得的培养物中含有许多形态功能特征与胰岛β细胞相对应的上皮细胞；在这些细胞的细胞质中发现了醛复红阳性颗粒。在营养培养基中葡萄糖浓度升高的作用下，这些细胞发生了脱颗粒。
