Wang Yeru, Cui Shenghui, Li Fengqin
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Wei Sheng Yan Jiu. 2008 Nov;37(6):685-9.
To detect and type Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
With the optimized experimental conditions, a total of 88 Salmonella strains isolated from feces of patients suffered from diarrhea were applied onto MALDI-TOF-MS and the results were compared with those obtained from serotype profiles, drug-resistance profiles and PFGE profiles.
The results illustrated that all strains tested were divided into 15 types by MALDI-TOF-MS at the distance level of 100. Eighty-eight strains were further break into 44 subtypes, based on the results of MALDI-TOF-MS combined with those of drug-resistance profiles. All strains were classified as 46 subtypes and 64 subtypes in combination MALDI-TOF-MS with serotype profiles and PFGE profiles, respectively. These reveal that MALDI-TOF-MS is a good method in Salmonella species typing.
The detection and identification of foodborne pathogenic bacteria by MALDI-TOF-MS is of good characteristics of stability and reproducibility. It is a powerful tool in detecting and identifying foodborne pathogenic bacteria.
采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）对沙门氏菌进行检测和分型。
在优化的实验条件下，将从腹泻患者粪便中分离出的88株沙门氏菌应用于MALDI-TOF-MS，并将结果与血清型谱、耐药谱和脉冲场凝胶电泳（PFGE）谱的结果进行比较。
结果表明，在距离水平为100时，所有测试菌株通过MALDI-TOF-MS被分为15种类型。基于MALDI-TOF-MS结果与耐药谱结果，88株菌株进一步细分为44个亚型。在MALDI-TOF-MS分别与血清型谱和PFGE谱结合时，所有菌株分别被分类为46个亚型和64个亚型。这些结果表明MALDI-TOF-MS是沙门氏菌分型的一种好方法。
利用MALDI-TOF-MS检测和鉴定食源性病原体具有良好的稳定性和可重复性。它是检测和鉴定食源性病原体的有力工具。
