Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666, USA.
Syst Appl Microbiol. 2011 Feb;34(1):63-8. doi: 10.1016/j.syapm.2010.11.009. Epub 2011 Jan 15.
Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) was evaluated as a technique to characterize strains of the nitrogen-fixing actinomycete Frankia. MALDI-TOF MS reliably distinguished 37 isolates within the genus Frankia and assigned them to their respective host infection groups, i.e., the Alnus/Casuarina and the Elaeagnus host infection groups. The assignment of individual strains to sub-groups within the respective host infection groups was consistent with classification based on comparative sequence analysis of nifH gene fragments, confirming the usefulness of MALDI-TOF MS as a rapid and reliable tool for the characterization of Frankia strains.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）被评估为一种用于表征固氮放线菌弗兰克氏菌菌株的技术。MALDI-TOF MS 能够可靠地区分弗兰克氏菌属内的 37 个分离株，并将其分配到各自的宿主感染组，即桤木/木麻黄和沙棘宿主感染组。将单个菌株分配到各自宿主感染组内的亚组与基于 nifH 基因片段的比较序列分析的分类一致，证实 MALDI-TOF MS 作为一种快速可靠的弗兰克氏菌菌株表征工具的有用性。
