Szewczuk Z, Kubik A, Gocka G, Wieczorek Z, Zimecki M, Janusz M
Institute of Chemistry, University of Wrocław, Poland.
Peptides. 1991 May-Jun;12(3):487-92. doi: 10.1016/0196-9781(91)90089-8.
To determine the role of consecutive amino acid residues in the immunoreactivity of the shortest active fragment of PRP, a series of analogs substituted by L-alanine in successive positions of the peptide chain was synthesized. The immunological investigations were carried out in several models: the immune response to T-cell dependent antigen, SRBC (in vivo and in vitro) and to T-cell independent antigen, PVP in vitro. The immunotropic action of the peptide was, in addition, verified with respect to thymocytes forming autologous rosettes. The results of these experiments revealed immunotropic activity (in all tests) of the analog containing alanine instead of proline in position 5 of PRP-pentapeptide.
为了确定连续氨基酸残基在朊蛋白最短活性片段免疫反应性中的作用，合成了一系列在肽链连续位置被L-丙氨酸取代的类似物。在几种模型中进行了免疫学研究：对T细胞依赖性抗原（体内和体外的绵羊红细胞）以及对T细胞非依赖性抗原（体外的聚乙烯吡咯烷酮）的免疫反应。此外，还针对形成自身玫瑰花结的胸腺细胞验证了该肽的免疫促进作用。这些实验结果显示，在朊蛋白五肽的第5位含有丙氨酸而非脯氨酸的类似物具有免疫促进活性（在所有测试中）。
