小肠蔗糖酶-异麦芽糖酶复合物解离为具有酶活性的亚基。

Dissociation of small-intestinal sucrase - isomaltase complex into enzymatically active subunits.

Braun H, Cogoli A, Semenza G

Eur J Biochem. 1975 Apr 1;52(3):475-80. doi: 10.1111/j.1432-1033.1975.tb04016.x.

Abstract

The sucrase - isomaltase complex from rabbit small intestine dissociated into its subunits upon reaction with citraconic anhydride. They can recombine after deacylation under mild acidic conditions. 2. When citraconylated, the subunits could be separated and isolated in a catalytically active form. 3. The previously reported procedure for separation of the subunits by alkaline treatment at pH 9.6 is apparently not due to contaminating degradative enzymes (possibly still present at undetectable levels in the isolated sucrase - isomaltase complex) but to the action of alkali.

摘要

兔小肠中的蔗糖酶-异麦芽糖酶复合物与柠康酸酐反应后会解离成亚基。在温和酸性条件下脱酰基后它们可以重新组合。2. 柠康酰化后，亚基可以以催化活性形式分离和纯化。3. 先前报道的在pH 9.6下通过碱性处理分离亚基的方法显然不是由于污染性降解酶（在分离的蔗糖酶-异麦芽糖酶复合物中可能仍以不可检测的水平存在）的作用，而是由于碱的作用。