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由古菌ExoIII同源物Mth212通过直接链切割启动的DNA尿嘧啶修复。

DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision.

作者信息

Schomacher Lars, Chong James P J, McDermott Paul, Kramer Wilfried, Fritz Hans-Joachim

机构信息

Abteilung Molekulare Genetik und Präparative Molekularbiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.

出版信息

Nucleic Acids Res. 2009 Apr;37(7):2283-93. doi: 10.1093/nar/gkp102. Epub 2009 Feb 24.

DOI:10.1093/nar/gkp102
PMID:19240141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2673441/
Abstract

No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus DeltaH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus DeltaH has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus DeltaH.

摘要

嗜热栖热甲烷杆菌DeltaH的基因组中不存在UDG超家族中任何已知尿嘧啶DNA糖基化酶的基因，因此很难想象该生物体中DNA-U修复是如何启动的。最近，嗜热栖热甲烷杆菌DeltaH的ExoIII同源物Mth212已被鉴定为一种DNA尿苷内切核酸酶，这提示了DNA尿嘧啶修复可能存在一种新的内切核酸酶作用进入机制。由于没有可用的基因实验系统，该问题通过生物化学方法进行研究。由粗细胞提取物促进的体外DNA尿嘧啶修复测定明确证实了该机制确实在嗜热栖热甲烷杆菌DeltaH中起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/b670675e3aee/gkp102f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/fc228686ea06/gkp102f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/64ca14702860/gkp102f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/ddc483a1525d/gkp102f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/b670675e3aee/gkp102f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/fc228686ea06/gkp102f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/64ca14702860/gkp102f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/ddc483a1525d/gkp102f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/406f/2673441/b670675e3aee/gkp102f3.jpg

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