Kibbe Warren A
Robert H. Lurie Comprehensive Cancer Center, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W43-6. doi: 10.1093/nar/gkm234. Epub 2007 Apr 22.
We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar 'calculator' look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900,000 accesses of OligoCalc from nearly 200,000 distinct hosts, excluding search engines. OligoCalc is available at http://basic.northwestern.edu/biotools/OligoCalc.html, with links to the full source code, usage patterns and statistics at that link as well.
我们开发了OligoCalc，它是一个基于客户端的可通过网络访问的计算引擎，用于报告DNA和RNA单链及双链的特性，包括分子量、溶液浓度、解链温度、估计的吸光系数、分子间自互补性估计以及分子内发夹环形成。OligoCalc具有常见的“计算器”外观和感觉，使其易于理解和使用。OligoCalc纳入了三种计算寡核苷酸解链温度的常用方法，包括用于解链温度的最近邻热力学模型。自1997年首次上线以来，除搜索引擎外，来自近20万个不同主机的对OligoCalc的访问量已超过90万次。可通过http://basic.northwestern.edu/biotools/OligoCalc.html访问OligoCalc，该链接还提供完整源代码、使用模式和统计信息的链接。
