Stewart R J, Pesavento P A, Woerpel D N, Goldstein L S
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8470-4. doi: 10.1073/pnas.88.19.8470.
Recent evidence has suggested that the principal polypeptide component of the microtubule motor protein kinesin may be a member of an extended superfamily of related motor proteins. To gain insight into how large the kinesin superfamily might be and to begin determining the potential functions in which various superfamily members might participate, we identified and partially characterized six additional members of the Drosophila kinesin superfamily. Genes encoding these proteins were identified by using the polymerase chain reaction with degenerate primers corresponding to highly conserved regions of the kinesin heavy-chain motor domain. Partial sequencing of the six genes revealed that they encode proteins that are 40-60% identical to the motor domain of the kinesin heavy-chain sequence. The cytogenetic locations as well as the developmental and tissue-specific expression patterns have been determined. The data suggest that each of these six kinesin-like proteins may have functions in a wide variety of cell types and tissues.
最近有证据表明，微管运动蛋白驱动蛋白的主要多肽成分可能是一个相关运动蛋白的扩展超家族的成员。为了深入了解驱动蛋白超家族可能有多大，并开始确定各种超家族成员可能参与的潜在功能，我们鉴定并部分表征了果蝇驱动蛋白超家族的另外六个成员。通过使用聚合酶链反应和与驱动蛋白重链运动结构域高度保守区域相对应的简并引物来鉴定编码这些蛋白质的基因。对这六个基因的部分测序表明，它们编码的蛋白质与驱动蛋白重链序列的运动结构域有40%-60%的同一性。已经确定了它们的细胞遗传学位置以及发育和组织特异性表达模式。数据表明，这六种类驱动蛋白中的每一种可能在多种细胞类型和组织中发挥作用。
