Giniger Edward, Wells William, Jan Lily Yeh, Jan Yuh Nung
Howard Hughes Medical Institute and Departments of Biochemistry and Biophysics and of Physiology, Rm U-426, University of California, San Francisco, 94143, San Francisco, CA, USA.
Rouxs Arch Dev Biol. 1993 Mar;202(2):112-122. doi: 10.1007/BF00636536.
We have analyzed the development of neuronal projections inDrosophila by fusing the gene encodingDrosophila kinesin, a microtubule-associated motor protein, toEscherichia coli lacZ, and employing the resulting chimeric protein as a reporter molecule for labelling cells by the "enhancer-trap" method. Expression of kinesin-β-galactosidase in neurons has afforded a detailed view of the morphologies and projections of neurons. The images of cells provided by this method will facilitate anatomical and genetic investigations of theDrosophila nervous system as well as other cell types.
我们通过将编码果蝇驱动蛋白（一种与微管相关的运动蛋白）的基因与大肠杆菌β-半乳糖苷酶融合，并使用所得的嵌合蛋白作为报告分子，通过“增强子捕获”方法标记细胞，来分析果蝇中神经元投射的发育情况。神经元中驱动蛋白-β-半乳糖苷酶的表达提供了神经元形态和投射的详细视图。这种方法提供的细胞图像将有助于对果蝇神经系统以及其他细胞类型进行解剖学和遗传学研究。
