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柳枝稷(Panicum virgatum L.)中甾体皂苷的分离、表征及定量分析。

Isolation, characterization, and quantification of steroidal saponins in switchgrass (Panicum virgatum L.).

作者信息

Lee Stephen T, Mitchell Robert B, Wang Zhirui, Heiss Christian, Gardner Dale R, Azadi Parastoo

机构信息

US Department of Agriculture, Poisonous Plant Research Laboratory, Agricultural Research Service, Logan, Utah 84341, USA.

出版信息

J Agric Food Chem. 2009 Mar 25;57(6):2599-604. doi: 10.1021/jf803907y.

DOI:10.1021/jf803907y
PMID:19243100
Abstract

Switchgrass (Panicum virgatum L.) has been identified for development into an efficient and environmentally friendly biomass energy crop. A recent 5 year study demonstrated that switchgrass grown for biofuel production produced 540% more energy than what is needed to grow, harvest, and process it into cellulosic ethanol. If switchgrass is grown on a scale useful for a bioenergy source, some of the material could be used by livestock as hay or pasture. Switchgrass has been reported to cause hepatogenous photosensitization in lambs (Ovis aries) and horses (Equus caballus). In this study, a simple extraction and rapid reversed phase high-performance liquid chromatography-electrospray ionization-mass spectrometry method was developed for quantifying the major saponins in switchgrass samples. Differences in the relative concentrations of different saponins were observed between switchgrass cultivars and plant parts.

摘要

柳枝稷(Panicum virgatum L.)已被确定为一种高效且环保的生物质能源作物。最近一项为期5年的研究表明,用于生物燃料生产的柳枝稷所产生的能量比种植、收获并将其加工成纤维素乙醇所需的能量多540%。如果柳枝稷以对生物能源有用的规模种植,部分材料可被牲畜用作干草或牧草。据报道,柳枝稷会导致羔羊(Ovis aries)和马(Equus caballus)发生肝源性光敏反应。在本研究中,开发了一种简单的提取方法以及快速反相高效液相色谱 - 电喷雾电离 - 质谱法,用于定量柳枝稷样品中的主要皂苷。观察到柳枝稷品种和植物部位之间不同皂苷的相对浓度存在差异。

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