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动脉粥样硬化：兔模型血管壁的对比增强磁共振成像——钆弗塞特与钆喷酸葡胺的比较

Atherosclerosis: contrast-enhanced MR imaging of vessel wall in rabbit model--comparison of gadofosveset and gadopentetate dimeglumine.

作者信息

Lobbes Marc B I, Miserus Robbert-Jan J H M, Heeneman Sylvia, Passos Valeria Lima, Mutsaers Peter H A, Debernardi Nicola, Misselwitz Bernd, Post Mark, Daemen Mat J A P, van Engelshoven Jos M A, Leiner Tim, Kooi Marianne E

机构信息

Department of Radiology, Cardiovascular Research Institute Maastricht, Maastricht University Hospital, P. Debyeplein 25, PO Box 5800, 6202 AZ Maastricht, The Netherlands.

出版信息

Radiology. 2009 Mar;250(3):682-91. doi: 10.1148/radiol.2503080875.

DOI:10.1148/radiol.2503080875

PMID:19244042

Abstract

PURPOSE

To investigate the potential of gadofosveset for contrast material-enhanced magnetic resonance (MR) imaging of plaque in a rabbit model of atherosclerosis.

MATERIALS AND METHODS

All experiments were approved by the animal ethics committee. Thirty-one New Zealand White rabbits were included in one of four study groups: animals with atherosclerosis imaged with gadofosveset (n = 10) or gadopentetate dimeglumine (n = 7) and control animals imaged with gadofosveset (n = 7) or gadopentetate dimeglumine (n = 7). Aortic atherosclerosis was induced through endothelial denudation combined with a cholesterol-enriched diet. Control rabbits underwent a sham surgical procedure and received a regular diet. After 8 weeks, pre- and postcontrast T1-weighted MR images of the aortic vessel wall were acquired. Relative signal enhancement was determined with dedicated software. Statistical analysis was performed by using a generalized linear mixed model. Immunohistochemical staining with CD31 and albumin was used to assess microvessel density and the albumin content of the vascular wall. Group differences were analyzed by using a chi(2) test. Gadofosveset spatial distribution and content within the vessel wall were determined with proton-induced x-ray emission (PIXE) analysis.

RESULTS

Postcontrast signal enhancement was significantly greater for atherosclerotic than for control animals imaged with gadofosveset (P = .022). Gadopentetate dimeglumine could not enable discrimination between normal and atherosclerotic vessel walls (P = .428). PIXE analysis showed higher amounts of gadopentetate dimeglumine than gadofosveset in both atherosclerotic and normal rabbit aortas. Immunohistochemical staining revealed the presence of albumin and increased microvessel density in the vascular walls of atherosclerotic rabbits.

CONCLUSION

These results suggest that gadofosveset can be used to differentiate between atherosclerotic and normal rabbit vessel walls.

SUPPLEMENTAL MATERIAL

http://radiology.rsnajnls.org/cgi/content/full/250/3/682/DC1.

摘要

目的

在兔动脉粥样硬化模型中研究钆弗塞特用于斑块对比剂增强磁共振（MR）成像的潜力。

材料与方法

所有实验均经动物伦理委员会批准。31只新西兰白兔被纳入四个研究组之一：用钆弗塞特成像的动脉粥样硬化动物（n = 10）或钆喷酸葡胺成像的动脉粥样硬化动物（n = 7），以及用钆弗塞特成像的对照动物（n = 7）或钆喷酸葡胺成像的对照动物（n = 7）。通过内皮剥脱联合高胆固醇饮食诱导主动脉粥样硬化。对照兔接受假手术并给予常规饮食。8周后，采集主动脉血管壁的对比剂注射前和注射后的T1加权MR图像。使用专用软件确定相对信号增强。采用广义线性混合模型进行统计分析。用CD31和白蛋白进行免疫组织化学染色，以评估微血管密度和血管壁白蛋白含量。采用卡方检验分析组间差异。用质子诱导X射线发射（PIXE）分析确定钆弗塞特在血管壁内的空间分布和含量。

结果

用钆弗塞特成像时，动脉粥样硬化动物对比剂注射后的信号增强显著大于对照动物（P = .022）。钆喷酸葡胺无法区分正常和动脉粥样硬化血管壁（P = .428）。PIXE分析显示，在动脉粥样硬化和正常兔主动脉中，钆喷酸葡胺的含量均高于钆弗塞特。免疫组织化学染色显示动脉粥样硬化兔血管壁存在白蛋白且微血管密度增加。