范可尼贫血和布卢姆综合征DNA损伤反应网络中涉及的新蛋白质的鉴定与分析。
Identification and analysis of new proteins involved in the DNA damage response network of Fanconi anemia and Bloom syndrome.
作者信息
Guo Rong, Xu Dongyi, Wang Weidong
机构信息
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, MD 21224, USA.
出版信息
Methods. 2009 May;48(1):72-9. doi: 10.1016/j.ymeth.2009.02.011. Epub 2009 Feb 24.
Abstract
The use of co-immunoprecipitation (co-IP) to purify multi-protein complexes has contributed greatly to our understanding of the DNA damage response network associated with Fanconi anemia (FA), Bloom syndrome (BS) and breast cancer. Four new FA genes and two new protein partners for the Bloom syndrome gene product have been identified by co-IP. Here, we discuss our experience in using co-IP and other techniques to isolate and characterize new FA and BS-related proteins.
摘要
运用免疫共沉淀（co-IP）技术纯化多蛋白复合物，极大地增进了我们对与范可尼贫血（FA）、布卢姆综合征（BS）及乳腺癌相关的DNA损伤反应网络的理解。通过免疫共沉淀已鉴定出4个新的范可尼贫血基因以及布卢姆综合征基因产物的2个新的蛋白伙伴。在此，我们将讨论运用免疫共沉淀及其他技术分离和鉴定与范可尼贫血及布卢姆综合征相关新蛋白的经验。
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