[全反式维甲酸和他扎罗汀对热休克后人成纤维细胞中MMP-1和TIMP-1表达的影响]
[Effects of all-trans retinioic acid and tazarotene on MMP-1 and TIMP-1 expression in cultured human fibroblasts after heat shock].
作者信息
Liu Ping, Tan Sheng-shun, Wang Qiong-yu, Wang Yong-xian
机构信息
Department of Dermatology, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Feb;29(2):217-9.
OBJECTIVE
To investigate the molecular mechanism of dermal damage in heat shock-induced skin aging by observing the expressions of metalloproteinase-1 (MMP-1) and tissue inhibitor of MMP-1 (TIMP-1) in retinoic acid-treated cultured human fibroblasts with heat shock.
METHODS
Cultured human fibroblasts were treated with tazarotene or all-trans-retinioic acid (at-RA) after heat shock for 30 min in 43 degrees celsius; water bath. Twenty-four hours later, MMP-1 and TIMP-1 contents in the supernatant of the cell culture medium were measured using enzyme-linked immunosorbent assay (ELISA).
RESULTS
Both tazarotene and at-RA dose-dependently reduced the expression of MMP-1 and increased the expression of TIMP-1 in cultured human fibroblasts exposed to heat shock, and tazarotene produced stronger effect than at-RA.
CONCLUSION
Retinoic acid can reduce the expression of MMP-1 and increase the expression of TIMP-1 in cultured human fibroblasts, suggesting its therapeutic potential for heat shock-induced skin aging.
目的
通过观察维甲酸处理的热休克培养人成纤维细胞中基质金属蛋白酶-1（MMP-1）和MMP-1组织抑制剂（TIMP-1）的表达，探讨热休克诱导皮肤老化中皮肤损伤的分子机制。
方法
在43摄氏度水浴中热休克30分钟后，用他扎罗汀或全反式维甲酸（at-RA）处理培养的人成纤维细胞。24小时后，采用酶联免疫吸附测定（ELISA）法检测细胞培养基上清液中MMP-1和TIMP-1的含量。
结果
他扎罗汀和at-RA均剂量依赖性地降低热休克培养人成纤维细胞中MMP-1的表达并增加TIMP-1的表达，且他扎罗汀的作用强于at-RA。
结论
维甲酸可降低培养人成纤维细胞中MMP-1的表达并增加TIMP-1的表达，提示其对热休克诱导的皮肤老化具有治疗潜力。
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