牛带绦虫囊尾蚴盐水提取物作为人类脑脊液中神经囊尾蚴病免疫诊断的替代抗原。
Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid.
作者信息
Oliveira Heliana B, Machado Gleyce A, Gonçalves-Pires Maria do Rosário F, Moura Leandro P, Costa-Cruz Julia M
机构信息
Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Minas Gerais, Brazil.
出版信息
Parasitol Res. 2009 Jul;105(1):169-74. doi: 10.1007/s00436-009-1379-z. Epub 2009 Feb 27.
The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.
本研究的目的是测试牛带绦虫囊尾蚴作为一种替代抗原，用于酶联免疫吸附测定（ELISA）和蛋白质印迹法（WB）检测，并与猪带绦虫囊尾蚴抗原用于脑脊液（CSF）样本检测进行比较。样本取自35例确诊为神经囊尾蚴病（NCC）的患者（第1组）和44例患有其他神经系统疾病的患者（对照组，第2组）。使用从猪带绦虫获得的抗原进行ELISA检测，应用于第1组患者时，敏感性和特异性结果均为100%。当使用牛带绦虫囊尾蚴进行检测时，结果分别为100%和93.2%。当用两种抗原进行WB检测时，47 - 52 kDa、64 - 68 kDa和70 kDa抗原在NCC患者的CSF样本中出现频率较高。结果表明，牛带绦虫囊尾蚴可作为CSF样本中人类NCC诊断的替代抗原。
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