da Silva Gabriela B, Nunes Daniela S, de Sousa José Eduardo N, Gonçalves-Pires Maria do R F, Levenhagen Marcelo A, Costa-Cruz Julia M
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
Parasitol Int. 2017 Apr;66(2):134-138. doi: 10.1016/j.parint.2016.12.009. Epub 2016 Dec 21.
This study aimed to evaluate the total extract of Taenia crassiceps metacestodes (TC) and its antigenic fractions obtained by Triton X-114 fractionation techniques, such as detergent (DC) and aqueous (AC), in the immunodiagnosis of human neurocysticercosis (NCC). Cerebrospinal fluid samples were divided into two groups: Group 1 (n=40), which was further divided into active (n=20) and inactive (n=20) NCC, and Group 2 (control group), which comprised 39 CSF samples from patients who had another neurological disorder, were suffering from other infectious diseases of the brain or had other parasitic infections. The total extracts and antigenic fractions were tested by enzyme-linked immunosorbent assay (ELISA) to detect human IgG anti-Taenia solium. T. crassiceps fractions (DC and AC) showed the same value of sensitivity (Se), 100%, for active and inactive NCC and a specificity (Sp) of 97.4%. The DS fraction obtained from T. solium showed 100% Se for active NCC, 95% Se for inactive NCC and a 92.3% Sp. The AS fraction obtained from T. solium showed 100% Se for both active and inactive NCC and a 94.9% Sp. There was a positive correlation between the total saline extract of T. crassiceps (TC) and T. solium (TS) and their fractions (DC, AC, DS and AS). Positive predictive value, negative predictive value, diagnostic efficiency and Youden index were calculated. In conclusion, these results demonstrated that detergent and aqueous fractions obtained from T. crassiceps metacestodes are important sources of specific antigens and are efficient for immunodiagnosis of active and inactive NCC.
本研究旨在评估肥胖带绦虫囊尾蚴的总提取物（TC）及其通过Triton X - 114分级分离技术获得的抗原组分，即去污剂组分（DC）和水性组分（AC），在人类神经囊尾蚴病（NCC）免疫诊断中的作用。脑脊液样本分为两组：第1组（n = 40），进一步分为活动性NCC组（n = 20）和非活动性NCC组（n = 20）；第2组为对照组，包含39例患有其他神经系统疾病、脑部其他传染病或其他寄生虫感染患者的脑脊液样本。通过酶联免疫吸附测定（ELISA）检测总提取物和抗原组分，以检测人抗猪带绦虫IgG。肥胖带绦虫组分（DC和AC）对活动性和非活动性NCC的敏感性（Se）值相同，均为100%，特异性（Sp）为97.4%。猪带绦虫的去污剂可溶性组分（DS）对活动性NCC的Se为100%，对非活动性NCC的Se为95%，Sp为92.3%。猪带绦虫的水性可溶性组分（AS）对活动性和非活动性NCC的Se均为100%，Sp为94.9%。肥胖带绦虫（TC）和猪带绦虫（TS）的总盐提取物及其组分（DC、AC、DS和AS）之间存在正相关。计算了阳性预测值、阴性预测值、诊断效率和尤登指数。总之，这些结果表明，从肥胖带绦虫囊尾蚴获得的去污剂和水性组分是特异性抗原的重要来源，对活动性和非活动性NCC的免疫诊断有效。
