Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, MG, Brazil.
Diagn Microbiol Infect Dis. 2010 Nov;68(3):259-64. doi: 10.1016/j.diagmicrobio.2010.06.016. Epub 2010 Sep 16.
The aim of this study was to evaluate jacalin-bound fraction (JBF) and jacalin-unbound fraction (JUF) of the total saline extract from Taenia saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose(®)-jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39-42, 47-52, 64-68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
本研究旨在评估猪带绦虫囊尾蚴总盐水提取物中亲和层析分离得到的荆豆凝集素结合部分（JBF）和荆豆凝集素未结合部分（JUF）在脑脊液中用于人类脑囊尾蚴病（NC）免疫诊断的效果。使用 Sepharose®-荆豆凝集素通过亲和层析法分离总提取物、JBF 和 JUF，并在酶联免疫吸附试验（ELISA）和 Western 印迹（WB）中进行测试，以检测免疫球蛋白 G。在 ELISA 试验中，JUF 分别显示出 92%和 100%的更高诊断效率和特异性指数。在 WB 中，使用 JUF 检测到 5 种免疫优势蛋白（39-42、47-52、64-68、70 和 75 kDa）。综上所述，研究结果表明，从猪带绦虫囊尾蚴中获得的 JUF 是特异性肽的重要来源，并且在 NC 的诊断中非常有效。
