Ota Masaki, Kato Yoshitaka, Watanabe Hiromoto, Watanabe Masaru, Sato Yoshiyuki, Smith Richard L, Inomata Hiroshi
Research Center of Supercritical Fluid Technology, Tohoku University, Aramaki Aza Aoba-6-6-11, Aoba-ku, Sendai 980-8579, Japan.
Biotechnol Prog. 2009 Mar-Apr;25(2):492-8. doi: 10.1002/btpr.123.
The growth rate of a highly CO(2)-tolerant green alga, Chlorococcum littorale, was investigated in semi-batch cultures at a temperature of 22 degrees C, a light intensity of 170 micromol-photon m(-2) s(-1) and CO(2) concentrations ranging from 1 to 50% (v/v) at atmospheric pressure. In the experiments, solutions were bubbled with CO(2) and N(2) gas mixtures to adjust CO(2) concentrations to minimize the influence of O(2). Growth rate, which was defined in terms of a specific growth rate mu, decreased with increasing CO(2) concentration at the conditions studied. The inhibition of growth by CO(2) gas could be attributed to the concentration of inorganic carbon in the culture medium. A growth model is proposed where key assumptions are the formation of bicarbonate ion HCO(3) (-) as substrate for algal growth and equilibrium between CO(2) inhibitor. The proposed growth model based on the Monod equation agreed with the experimental data to within 5% and provides better correlation than the conventional inhibition model, especially in the high CO(2) concentration region.
在22摄氏度的温度、170微摩尔光子·米⁻²·秒⁻¹的光照强度以及大气压下1%至50%（v/v）的二氧化碳浓度条件下，采用半连续培养法研究了一种高度耐二氧化碳的绿藻——滨海绿球藻的生长速率。实验中，用二氧化碳和氮气混合气体对溶液进行鼓泡，以调节二氧化碳浓度，从而将氧气的影响降至最低。在所研究的条件下，以比生长速率μ定义的生长速率随二氧化碳浓度的增加而降低。二氧化碳气体对生长的抑制作用可能归因于培养基中无机碳的浓度。提出了一个生长模型，其关键假设是形成碳酸氢根离子HCO₃⁻作为藻类生长的底物以及二氧化碳抑制剂之间的平衡。基于莫诺德方程提出的生长模型与实验数据的吻合度在5%以内，并且比传统的抑制模型具有更好的相关性，尤其是在高二氧化碳浓度区域。
