Nano Optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
Bioprocess Biosyst Eng. 2010 Jan;33(1):133-9. doi: 10.1007/s00449-009-0362-5. Epub 2009 Aug 7.
A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO(2)-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 micromol photon/m(2)/s, while keeping the other conditions in the CO(2)-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.
采用 CO2 进料平板式光生物反应器，在非同步光照条件下，建立了富含虾青素的雨生红球藻高密度培养的分批补料培养工艺，随后进行光自养诱导。采用指数补料策略，补充生长限制营养物硝酸盐和磷酸盐，同时根据细胞浓度逐步补充光，进行分批补料培养。在生长阶段，获得了 1.47 g/L 的生物量，生物量生产率为 0.33 g/L/天。通过将光照强度增加到 400 μmol 光子/m2/s，连续对生长良好的营养细胞进行光自养诱导，同时保持 CO2 进料平板式光生物反应器中的其他条件不变，得到了 190 mg/L 的虾青素产量，虾青素生产率为 14 mg/L/天。所提出的顺序光自养工艺具有作为生产有价值的雨生红球藻虾青素的简单高效工艺的巨大潜力。
