Lécuyer Eric, Yoshida Hideki, Krause Henry M
Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario, Canada M5S 3E1.
Curr Opin Cell Biol. 2009 Jun;21(3):409-15. doi: 10.1016/j.ceb.2009.01.027. Epub 2009 Feb 25.
Genome expression profiling has led to the important realization that RNA molecules are more numerous and diverse than previously expected. One aspect of RNA biology that is just beginning to be fully appreciated is the extent to which mRNAs are transported to specific subcellular destinations before being translated, an exquisite mechanism for targeting proteins where they are required in the cell. While several excellent reviews have discussed the subject of mRNA localization, it is only in recent years that high-throughput technologies have been applied to address issues such as the extent and diversity of RNA localization events and mechanisms. This review focuses on these recent functional genomic approaches, their implications, and the new tools and methods that will be needed to further elucidate mRNA localization pathways.
基因组表达谱分析使人们重要地认识到，RNA分子比以前预期的数量更多、种类更丰富。RNA生物学中一个刚刚开始得到充分认识的方面是，mRNA在翻译之前被转运到特定亚细胞定位的程度，这是一种在细胞中需要蛋白质的地方靶向蛋白质的精妙机制。虽然已有几篇优秀的综述讨论了mRNA定位的主题，但直到近年来，高通量技术才被应用于解决诸如RNA定位事件和机制的程度及多样性等问题。本综述重点关注这些最新的功能基因组学方法、它们的意义以及进一步阐明mRNA定位途径所需的新工具和方法。
