Low Suet P, Voelcker Nicolas H, Canham Leigh T, Williams Keryn A
Flinders University, Adelaide, SA, Australia.
Biomaterials. 2009 May;30(15):2873-80. doi: 10.1016/j.biomaterials.2009.02.008. Epub 2009 Feb 28.
In this report, we explore the biocompatibility of thermally-oxidised, aminosilanised porous silicon membranes and their potential to support human ocular cells in vitro and in vivo, in the rat eye. A colorimetric assay for silicic acid showed that membranes with pore sizes of 40-60nm slowly dissolved, but the material could be maintained in tissue culture medium in vitro for at least two weeks without visible degradation. When implanted under the rat conjunctiva, the material did not erode the underlying or overlying tissue. The implant underwent slow dissolution, but remained visible at the operating microscope for over 8 weeks. End-stage histology indicated the presence of a thin fibrous capsule surrounding the implant, but little evidence of any local accumulation of acute inflammatory cells or vascularization. Human lens epithelial cells and primary human corneal explants adhered to the porous silicon membranes, where they remained viable and underwent division. Primary corneal epithelial cells supported on membranes were labelled with a cell tracker dye and implanted under the rat conjunctiva. Seven days later, labelled cells had moved from the membrane into the ocular tissue spaces. A porous silicon membrane may have value as a biomaterial that can support the delivery of cells to the ocular surface and improve existing therapeutic options in patients with corneal epithelial stem cell dysfunction and ocular surface disease.
在本报告中，我们探究了热氧化、氨基硅烷化多孔硅膜的生物相容性及其在体外和体内（大鼠眼内）支持人眼细胞的潜力。硅酸的比色测定表明，孔径为40 - 60nm的膜会缓慢溶解，但该材料在体外组织培养基中可维持至少两周而无明显降解。当植入大鼠结膜下时，该材料不会侵蚀下方或上方的组织。植入物会缓慢溶解，但在手术显微镜下8周多仍可见。终末期组织学检查表明植入物周围存在一层薄纤维囊，但几乎没有急性炎症细胞局部聚集或血管化的迹象。人晶状体上皮细胞和原代人角膜外植体附着在多孔硅膜上，并在其上保持存活并进行分裂。附着在膜上的原代角膜上皮细胞用细胞追踪染料标记后植入大鼠结膜下。七天后，标记细胞已从膜移入眼组织间隙。多孔硅膜作为一种生物材料，可能具有支持将细胞递送至眼表并改善角膜上皮干细胞功能障碍和眼表疾病患者现有治疗选择的价值。
