Zhang Mengxi, Chen Yuda, Chung Anna, Yang Shudan, Choi Chi Hun, Zhang Sophie, Han Yimo, Xiao Han
Department of Chemistry, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
The Awty International Schoo, 6100 Main Street, Houston, TX, 77055, USA.
Small Methods. 2024 Dec;8(12):e2400230. doi: 10.1002/smtd.202400230. Epub 2024 Sep 17.
3,4-Dihydroxy-L-phenylalanine (DOPA) serves as a post-translational modification amino acid present in mussel foot proteins. Mussels exploit the exceptional adhesive properties of DOPA to adhere to a wide range of surfaces. This study presents the development of sticky proteins and bacteria through the site-specific incorporation of DOPA using Genetic Code Expansion Technology. Through the optimization of the DOPA incorporation system, proteins containing DOPA demonstrate significantly improved binding abilities to various organic and metallic materials. The material-binding capabilities of DOPA to combat different types of biofoulings are harnessed by integrating it into intrinsically disordered proteins. Beyond the creation of adhesive proteins for anti-biofouling purposes, this highly efficient DOPA incorporation system is also applied to engineer adhesive bacteria, resulting in a remarkable increase in their binding capability to diverse materials including 400 folds of improvement to polyethylene terephthalate (PET). This substantial enhancement in PET binding of these bacteria has allowed to develop a unique approach for PET degradation, showcasing the innovative application of Genetic Code Expansion in cell engineering.
3,4-二羟基-L-苯丙氨酸（多巴）是贻贝足蛋白中存在的一种翻译后修饰氨基酸。贻贝利用多巴卓越的粘附特性附着在各种表面上。本研究展示了通过遗传密码扩展技术位点特异性掺入多巴来开发粘性蛋白质和细菌。通过优化多巴掺入系统，含多巴的蛋白质对各种有机和金属材料的结合能力显著提高。通过将多巴整合到内在无序蛋白质中，利用多巴对抗不同类型生物污垢的材料结合能力。除了创建用于抗生物污垢目的的粘附蛋白外，这种高效的多巴掺入系统还应用于工程化粘附细菌，使其对包括聚对苯二甲酸乙二酯（PET）在内的多种材料的结合能力显著提高，对PET的结合能力提高了400倍。这些细菌对PET结合能力的大幅增强使得开发出一种独特的PET降解方法成为可能，展示了遗传密码扩展在细胞工程中的创新应用。
