体内诱导的RTX毒素ApxIVA对于胸膜肺炎放线杆菌的完全毒力至关重要。
In vivo induced RTX toxin ApxIVA is essential for the full virulence of Actinobacillus pleuropneumoniae.
作者信息
Liu Jinlin, Chen Xia, Tan Chen, Guo Yi, Chen Yan, Fu Shulin, Bei Weicheng, Chen Huanchun
机构信息
Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
出版信息
Vet Microbiol. 2009 Jun 12;137(3-4):282-9. doi: 10.1016/j.vetmic.2009.01.011. Epub 2009 Jan 8.
Actinobacillus pleuropneumoniae is a Gram-negative pathogen. It is the aetiological agent of porcine contagious pleuropneumonia (PCP), a severe and highly contagious and severe respiratory disease of swine. Four sets of RTX (repeats in toxin) exotoxins have been described in A. pleuropnuemoniae and three of them have been characterized as important virulence determinants. The aim of this study was to determine the pathogenicity of the invivo induced RTX toxin ApxIVA during infection of piglets with A. pleuropnuemoniae. An A. pleuropnuemoniae apxIVA mutant was obtained based on an A. pleuropnuemoniae apxIIC-deleted mutant strain. An experimental infection assay was performed to evaluate the virulence of ApxIVA in piglets. Clinical signs, lung lesion scores, blood biochemical parameters and histopathologic changes in the piglets were recorded. The results indicated that the pathogenicity of A. pleuropnuemoniae was greater when ApxIVA was present, suggesting that ApxIVA is essential for expression of the full virulence of A. pleuropnuemoniae.
胸膜肺炎放线杆菌是一种革兰氏阴性病原体。它是猪传染性胸膜肺炎（PCP）的病原体，PCP是一种严重的、高度传染性的猪呼吸道疾病。在胸膜肺炎放线杆菌中已描述了四组RTX（毒素重复序列）外毒素，其中三组已被鉴定为重要的毒力决定因素。本研究的目的是确定在仔猪感染胸膜肺炎放线杆菌期间体内诱导的RTX毒素ApxIVA的致病性。基于胸膜肺炎放线杆菌apxIIC缺失突变株获得了胸膜肺炎放线杆菌apxIVA突变体。进行了实验性感染试验以评估ApxIVA在仔猪中的毒力。记录了仔猪的临床症状、肺损伤评分、血液生化参数和组织病理学变化。结果表明，当存在ApxIVA时，胸膜肺炎放线杆菌的致病性更强，这表明ApxIVA对于胸膜肺炎放线杆菌的完全毒力表达至关重要。
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