Gómez-Román Jose Javier, Martínez Montserrat Nicolás, Fernández Servando Lazuén, Val-Bernal Jose Fernando
Departamento de Anatomía Patológica, Hospital Universitario Marqués de Valdecilla Servicio Cántabro de Salud, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
Mod Pathol. 2009 Apr;22(4):530-7. doi: 10.1038/modpathol.2009.7. Epub 2009 Feb 27.
The association of Epstein-Barr virus with pulmonary neoplasms has been restricted to lymphoepithelioma-like carcinomas in Asian patients. We have selected 19 pulmonary adenocarcinomas and squamous-cell carcinomas from 1545 pulmonary neoplasms diagnosed from 1996 to 2007 in an occidental population. All of them showed a low-power appearance confusing between an epithelial and a lymphoid neoplasm, with a dense lymphocytic infiltrate intermingled with neoplastic cells giving an image akin to lymphoepithelial complexes. Five carcinomas presented typical features of Lymphoepithelioma-like lung carcinomas; but six cases could be classified as squamous-cell carcinomas and eight as adenocarcinomas. A semiquantitative polymerase chain reaction method, Early RNA genes 1 and 2 in situ hybridization as well as Latent membrane protein immunostaining for Epstein-Barr virus DNA, RNA and protein detection methods were used in every case. None of Lymphoepithelioma-like carcinomas showed positivity for Epstein-Barr virus in any used method. Otherwise four squamous-cell carcinomas and eight adenocarcinomas (12 cases) demonstrated viral sequences in polymerase chain reaction and/or in situ hybridization analysis in neoplastic cells. Moreover two adenocarcinomas also displayed human herpesvirus 6 DNA sequences coamplification in molecular analysis. Protein immunostaining was focally positive in only three cases. We performed the same analysis in 70 more cases of conventional pulmonary squamous-cell carcinomas and adenocarcinomas that gave negative results. In conclusion, a subset of pulmonary squamous-cell carcinomas and adenocarcinomas show Epstein-Barr DNA and/or RNA sequences in neoplastic cells. This finding expands the spectra of epithelial cell common tumours Epstein-Barr virus associated.
爱泼斯坦-巴尔病毒与肺部肿瘤的关联仅限于亚洲患者的淋巴上皮瘤样癌。我们从1996年至2007年在西方人群中诊断出的1545例肺部肿瘤中选取了19例肺腺癌和鳞状细胞癌。所有病例在低倍镜下均表现出上皮性肿瘤和淋巴样肿瘤难以区分的外观，密集的淋巴细胞浸润与肿瘤细胞混合，呈现出类似淋巴上皮复合体的图像。5例癌呈现出淋巴上皮瘤样肺癌的典型特征；但6例可归类为鳞状细胞癌，8例为腺癌。对每例病例均采用半定量聚合酶链反应方法、早期RNA基因1和2原位杂交以及针对爱泼斯坦-巴尔病毒DNA、RNA和蛋白质检测方法的潜伏膜蛋白免疫染色。在任何所使用的方法中，淋巴上皮瘤样癌均未显示出爱泼斯坦-巴尔病毒阳性。否则，4例鳞状细胞癌和8例腺癌（共12例）在肿瘤细胞的聚合酶链反应和/或原位杂交分析中显示出病毒序列。此外，在分子分析中，2例腺癌还显示出人类疱疹病毒6 DNA序列的共扩增。蛋白质免疫染色仅在3例中呈局灶性阳性。我们对另外70例常规肺鳞状细胞癌和腺癌进行了相同分析，结果均为阴性。总之，一部分肺鳞状细胞癌和腺癌在肿瘤细胞中显示有爱泼斯坦-巴尔病毒DNA和/或RNA序列。这一发现扩展了与爱泼斯坦-巴尔病毒相关的上皮细胞常见肿瘤的范围。
