Fabre Laurent, Inoue Yoshihiko, Aoki Takafumi, Kawakami Shojiro
Graduate School of Information Sciences, Tohoku University, 6-6-04 Aramaki Aza Aoba, Aoba-ku,Sendai, Miyagi 980-8579, Japan.
Appl Opt. 2009 Mar 1;48(7):1347-57. doi: 10.1364/ao.48.001347.
We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase two-dimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample's three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.
我们展示了一种使用光子晶体的微分干涉对比显微镜，它能够在没有移动部件的情况下独立实时捕获相位和振幅分量。与以往使用旋转偏振器来区分每个分量的方法不同，我们建议使用配备有阵列偏振器的特殊相机，其即时偏振测量允许实时获取相位梯度信息。利用双图像算法，通过透射型显微镜从梯度信息重建生物样品的相位二维分布。我们还讨论了如何推导反射型显微镜下样品的三维形状。实验证明了该方法的有效性。
