Picazo-Bueno José Ángel, Zalevsky Zeev, García Javier, Ferreira Carlos, Micó Vicente
Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
Bar-Ilan University, Faculty of Engineering, Ramat-Gan 52900, Israel.
J Biomed Opt. 2016 Oct 1;21(10):106007. doi: 10.1117/1.JBO.21.10.106007.
We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).
我们最近报道了一种将标准显微镜转换为全息显微镜的简单、低成本且高度稳定的方法[《光学快报》22, 14929 (2014)]。该方法名为空间复用干涉显微镜（SMIM），它提出了一种在常规（非全息）显微镜上实现的离轴全息架构，只需进行最小程度的修改：使用相干照明以及一个放置和选择得当的一维衍射光栅。在本论文中，我们报告了在SMIM中实施部分（时间上降低）相干照明作为改善定量相位成像的一种方法。使用低相干光源迫使我们应用相移算法而非离轴全息记录来恢复样品的相位信息，但由于相干噪声的降低，改善了相位重建。此外，与我们之前报道的方案（1/3）相比，实施了限制较少的视场限制（1/2）。考虑到多种样品（分辨率测试、微珠、猪精子细胞、红细胞和前列腺癌细胞），在常规的奥林巴斯BX - 60正置显微镜中对所提出的改进进行了实验验证。
