使用偏振相机的微分干涉对比显微镜进行视频速率定量相分析。
Video-rate quantitative phase analysis by a DIC microscope using a polarization camera.
作者信息
Shibata Shuhei, Takano Wataru, Hagen Nathan, Matsuda Masaru, Otani Yukitoshi
机构信息
Department of Optical Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
Center for Optical Research and Education, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
出版信息
Biomed Opt Express. 2019 Feb 19;10(3):1273-1281. doi: 10.1364/BOE.10.001273. eCollection 2019 Mar 1.
Abstract
This paper describes how to take advantage of the replacement of an intensity camera with a polarization camera in a standard differential interference contrast (DIC) microscope. Using a polarization camera enables snapshot quantitative phase analysis so that real-time imaging of living transparent tissues become possible. Using our method, we quantify the phase measurement accuracy using a phantom consisting of glass beads embedded in lacquer. In order to demonstrate these advantages, we image the pumping heart and blood flow in a living egg.
摘要
本文描述了如何在标准微分干涉对比（DIC）显微镜中利用偏振相机取代强度相机。使用偏振相机可实现快照定量相位分析，从而使对活体透明组织进行实时成像成为可能。使用我们的方法，我们使用由嵌入漆中的玻璃珠组成的体模来量化相位测量精度。为了证明这些优点，我们对活鸡蛋中的跳动心脏和血流进行成像。
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本文引用的文献
1
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Sci Rep. 2017 Mar 24;7:44034. doi: 10.1038/srep44034.
2
CCD polarization imaging sensor with aluminum nanowire optical filters.带有铝纳米线光学滤波器的电荷耦合器件偏振成像传感器。
Opt Express. 2010 Aug 30;18(18):19087-94. doi: 10.1364/OE.18.019087.
3
Nomarski differential interference contrast microscopy for surface slope measurements: an examination of techniques.用于表面斜率测量的诺马斯基微分干涉对比显微镜：技术研究
Appl Opt. 1981 Aug 1;20(15):2665-9. doi: 10.1364/AO.20.002665.
4
Quantitative surface topography determination by Nomarski reflection microscopy. 2: Microscope modification, calibration, and planar sample experiments.用诺马斯基反射显微镜进行定量表面形貌测定。2：显微镜改装、校准及平面样品实验。
Appl Opt. 1980 Sep 1;19(17):2998-3009. doi: 10.1364/AO.19.002998.
5
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Opt Lett. 2009 Oct 15;34(20):3187-9. doi: 10.1364/OL.34.003187.
6
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7
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