Shibata Shuhei, Takano Wataru, Hagen Nathan, Matsuda Masaru, Otani Yukitoshi
Department of Optical Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
Center for Optical Research and Education, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
Biomed Opt Express. 2019 Feb 19;10(3):1273-1281. doi: 10.1364/BOE.10.001273. eCollection 2019 Mar 1.
This paper describes how to take advantage of the replacement of an intensity camera with a polarization camera in a standard differential interference contrast (DIC) microscope. Using a polarization camera enables snapshot quantitative phase analysis so that real-time imaging of living transparent tissues become possible. Using our method, we quantify the phase measurement accuracy using a phantom consisting of glass beads embedded in lacquer. In order to demonstrate these advantages, we image the pumping heart and blood flow in a living egg.
本文描述了如何在标准微分干涉对比（DIC）显微镜中利用偏振相机取代强度相机。使用偏振相机可实现快照定量相位分析，从而使对活体透明组织进行实时成像成为可能。使用我们的方法，我们使用由嵌入漆中的玻璃珠组成的体模来量化相位测量精度。为了证明这些优点，我们对活鸡蛋中的跳动心脏和血流进行成像。
