Department of Operative Dentistry and Periodontology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
Clin Oral Investig. 2009 Dec;13(4):383-91. doi: 10.1007/s00784-009-0260-x. Epub 2009 Feb 28.
Recently, osteogenic precursor cells were isolated from human dental follicles, which differentiate into cementoblast- or osteoblast-like cells under in vitro conditions after the induction with dexamethasone or insulin. However, mechanisms for osteogenic differentiation are not understood in detail. In a previous study, real-time RT-PCR results demonstrated molecular mechanisms in dental follicle cells (DFCs) during osteogenic differentiation that are different from those in bone-marrow-derived mesenchymal stem cells. We analysed gene expression profiles in DFCs before and after osteogenic differentiation with the Affymetrix GeneChip(R) Human Gene 1.0 ST Array. Transcripts of 98 genes were up-regulated after differentiation. These genes could be clustered into subcategories such as cell differentiation, cell morphogenesis, and skeletal development. Osteoblast-specific transcription factors like osterix and runx2 were constitutively expressed in differentiated DFCs. In contrast, the transcription factor ZBTB16, which promotes the osteoblastic differentiation of mesenchymal stem cells as an up-stream regulator of runx2, was differentially expressed after differentiation. Transcription factors NR4A3, KLF9 and TSC22D3, involved in the regulation of cellular development, were up-regulated as well. In conclusion, we present the first transcriptome of human DFCs before and after osteogenic differentiation. This study sheds new light on the complex mechanism of osteogenic differentiation in DFCs.
最近，从人牙囊分离出成骨前体细胞，经地塞米松或胰岛素诱导后，在体外条件下可分化为成牙骨质细胞或成骨细胞样细胞。然而，其成骨分化的机制尚不清楚。在之前的研究中，实时 RT-PCR 结果表明，牙囊细胞（DFCs）在成骨分化过程中的分子机制与骨髓间充质干细胞不同。我们用 Affymetrix GeneChip(R) Human Gene 1.0 ST Array 分析了成骨分化前后 DFCs 的基因表达谱。分化后有 98 个基因的转录本上调。这些基因可以聚类为细胞分化、细胞形态发生和骨骼发育等亚类。成骨细胞特异性转录因子如osterix 和 runx2 在分化的 DFCs 中持续表达。相比之下，作为 runx2 的上游调节剂，促进间充质干细胞成骨分化的转录因子 ZBTB16 在分化后表达差异。参与细胞发育调节的转录因子 NR4A3、KLF9 和 TSC22D3 也上调。总之，我们首次展示了人牙囊成骨分化前后的转录组图谱。本研究为牙囊细胞成骨分化的复杂机制提供了新的见解。
