Azarpira Negar, Dehghani Mehdi, Aghdaie Mahdokth H, Darai Masumeh
Organ Transplant Research Center, Shiraz University of Medical Sciences, Zand Street, Nemazi Hospital, Shiraz, Iran.
Mol Biol Rep. 2010 Jan;37(1):27-31. doi: 10.1007/s11033-009-9488-4. Epub 2009 Mar 1.
Graft-versus-host disease is the main complication after hematopoietic stem cells transplantation (HSCT). Non-HLA genotypes, such as cytokines, have been investigated for their potential roles in the occurrence and severity of GVHD as well as for their contribution to overall transplant-related mortality and survival. IL-7 which is secreted by bone marrow stromal cells plays an important role in the development and survival of T cells. Its effect is mediated via interleukin 7 receptor (IL7R). This study investigates the possible links between IL-7alphaR single nucleotide polymorphisms (+510 C/T, +1237 A/G, +2087 T/C and +3110A/G) and transplant outcomes among 116 recipients of HSCT. Genotypes were determined using polymerase chain reaction-sequence-specific primers. No significant differences were observed between the genotypic distributions of IL-7alphaR polymorphisms and incidence of acute or chronic graft versus host disease. Additional studies with larger sample are necessary to further define the influence of IL-7alphaR on the immune response after bone marrow transplantation.
移植物抗宿主病是造血干细胞移植（HSCT）后的主要并发症。非HLA基因型，如细胞因子，已被研究其在移植物抗宿主病的发生和严重程度中的潜在作用，以及它们对总体移植相关死亡率和生存率的影响。由骨髓基质细胞分泌的白细胞介素-7在T细胞的发育和存活中起重要作用。其作用通过白细胞介素7受体（IL7R）介导。本研究调查了116例造血干细胞移植受者中白细胞介素-7α受体单核苷酸多态性（+510 C/T、+1237 A/G、+2087 T/C和+3110A/G）与移植结局之间的可能联系。使用聚合酶链反应-序列特异性引物确定基因型。在白细胞介素-7α受体多态性的基因型分布与急性或慢性移植物抗宿主病的发生率之间未观察到显著差异。需要进行更大样本量的进一步研究，以进一步确定白细胞介素-7α受体对骨髓移植后免疫反应的影响。
