Amitani R, Kawanami R
Department of Respiratory Medicine, Osaka Red Cross Hospital, Osaka, Japan.
Med Mycol. 2009;47 Suppl 1:S127-31. doi: 10.1080/13693780802558959. Epub 2009 Feb 27.
The interaction of Aspergillus fumigatus with human bronchial mucosa in an organ culture model, with an air-mucosal interface, was studied. A. fumigatus conidia were inoculated onto the organ culture tissues and incubated for 24 h. At each time point (1, 6, 12, 18 and 24 h), after the measurement of ciliary beat frequency (CBF) of bronchial epithelial cells, adherence and invasion of the epithelium by A. fumigatus conidia (and hyphae), as well as structural changes of the epithelium, were investigated by scanning and transmission electron microscopy. This study demonstrated that A. fumigatus caused damage to the bronchial epithelium that was associated with CBF slowing. In addition, the study demonstrated that some of the conidia of A. fumigatus were internalized within ciliated and non-ciliated epithelial cells, some conidia were located within the intercellular spaces of the epithelium, and that hyphae penetrated through both intercellular and intracellular spaces of the epithelium. These findings suggest there might be at least three pathways by which Aspergillus invades the bronchial mucosa: (1) penetration of hyphae through the intercellular spaces in the epithelium; (2) direct penetration of hyphae through epithelial cells; and (3) internalization of conidia within epithelial cells.
在具有气-黏膜界面的器官培养模型中，研究了烟曲霉与人支气管黏膜的相互作用。将烟曲霉分生孢子接种到器官培养组织上并孵育24小时。在每个时间点（1、6、12、18和24小时），在测量支气管上皮细胞的纤毛摆动频率（CBF）后，通过扫描电子显微镜和透射电子显微镜研究烟曲霉分生孢子（和菌丝）对上皮的黏附与侵袭以及上皮的结构变化。本研究表明，烟曲霉对支气管上皮造成损伤，这与CBF减慢有关。此外，该研究表明，一些烟曲霉分生孢子被内化到纤毛和非纤毛上皮细胞内，一些分生孢子位于上皮的细胞间隙内，并且菌丝穿过上皮的细胞间和细胞内空间。这些发现表明，烟曲霉侵入支气管黏膜可能至少有三种途径：（1）菌丝通过上皮中的细胞间隙穿透；（2）菌丝直接穿透上皮细胞；（3）分生孢子被上皮细胞内化。
