Varich N L, Gitel'man A K, Shilov A A, Smirnov Iu A, Kaverin N V
Vopr Virusol. 2009 Jan-Feb;54(1):7-11.
The gene composition of the viral population obtained via mixed infection of embryonated chick eggs with influenza viruses at a high multiplicity of infection was analyzed. In mixed infection caused by influenza A/WSN/33 (H1N1) and A/Duck/Czechoslovakia/56 (H4N6) viruses, the population showed a preponderance of the reassortants whose content of genomic segments originating from either of the parent virus deviated strongly from the random pattern: the hemagglutinin (HA) gene of A/WSN/33 (H1N1) virus and the NP gene of A/Duck/Czechoslovakia/56 (H4N6) virus were prevalent in the gene composition of the reassortants. The mixed infection produced by influenza A/Udorn/72 (H3N2) virus and the reassortant R8 containing the HA gene of A/Duck/Ho Chi Minh/014/78 (H5N3) virus, the population of reassortants contained mainly the HA gene of A/Udorn/72 (H3N2) virus and the NP gene of the reassortant R8. The findings are discussed due to the problem of specific recognition of gene segments when incorporated into the viral particles.
分析了通过以高感染复数用流感病毒混合感染鸡胚获得的病毒群体的基因组成。在由甲型流感病毒A/WSN/33（H1N1）和A/鸭/捷克斯洛伐克/56（H4N6）引起的混合感染中，该群体显示出重组体占优势，其源自任一亲本病毒的基因组片段含量与随机模式有很大偏差：A/WSN/33（H1N1）病毒的血凝素（HA）基因和A/鸭/捷克斯洛伐克/56（H4N6）病毒的NP基因在重组体的基因组成中占主导地位。由甲型流感病毒A/乌冬/72（H3N2）和含有A/鸭/胡志明市/014/78（H5N3）病毒HA基因的重组体R8产生的混合感染，重组体群体主要包含A/乌冬/72（H3N2）病毒的HA基因和重组体R8的NP基因。由于基因片段在掺入病毒颗粒时的特异性识别问题，对这些发现进行了讨论。
