Bannerman Douglas D, Rinaldi Manuela, Vinyard Bryan T, Laihia Jarmo, Leino Lasse
Bovine Functional Genomics Laboratory, USDA, Agricultural Research Service, Beltsville, MD, USA.
Am J Vet Res. 2009 Mar;70(3):373-82. doi: 10.2460/ajvr.70.3.373.
To evaluate the effects of cis-urocanic acid (cis-UCA) on mammary gland (MG) inflammation and injury associated with Escherichia coli-induced mastitis in dairy cows.
12 lactating dairy cows (36 MGs).
At 7-week intervals, a different MG in each cow was experimentally inoculated with E coli. At 6-hour intervals from 6 to 36 hours after inoculation, the inoculated MG in each cow was infused with 40 mL of saline (0.9% NaCl) solution, 12.5mM cis-UCA, or 25mM cis-UCA (4 cows/group); ultimately, each cow received each treatment. Immediately prior to and at various time points after inoculation and treatment, milk samples were collected. Bacterial CFUs, somatic cell counts (SCCs), N-acetyl-beta-D-glucosaminidase (NAGase) and lactate dehydrogenase (LDH) activities, and concentrations of bovine serum albumin, tumor necrosis factor-alpha, and cis-UCA were quantified in each milk sample. Results-Compared with findings in saline solution-treated MGs, NAGase and LDH activities in milk samples from cis-UCA-treated MGs were lower. Cis-UCA had no effect on milk SCCs and milk concentrations of bovine serum albumin and tumor necrosis factor-alpha. Furthermore, cis-UCA had no adverse effect on bacterial clearance; CFUs of E coli in MGs treated with saline solution or cis-UCA were equivalent.
In cows, milk NAGase and LDH activities were both lower in E coli-infected MGs infused with cis-UCA than in those infused with saline solution, which suggests that cis-UCA reduced mastitis-associated tissue damage. Furthermore, these data indicated that therapeutic concentrations of cis-UCA in milk can be achieved via intramammary infusion.
评估顺式尿刊酸（cis-UCA）对奶牛大肠杆菌诱导的乳腺炎相关乳腺（MG）炎症和损伤的影响。
12头泌乳奶牛（36个乳腺）。
每隔7周，对每头奶牛的不同乳腺进行大肠杆菌实验性接种。在接种后6至36小时，每隔6小时，对每头奶牛接种的乳腺注入40毫升生理盐水（0.9%氯化钠）溶液、12.5mM顺式尿刊酸或25mM顺式尿刊酸（每组4头奶牛）；最终，每头奶牛接受每种处理。在接种和处理前及各个时间点，立即采集牛奶样本。对每个牛奶样本中的细菌菌落形成单位（CFU）、体细胞计数（SCC）、N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）和乳酸脱氢酶（LDH）活性以及牛血清白蛋白、肿瘤坏死因子-α和顺式尿刊酸的浓度进行定量。结果：与生理盐水处理的乳腺相比，顺式尿刊酸处理的乳腺牛奶样本中的NAGase和LDH活性较低。顺式尿刊酸对牛奶SCC以及牛奶中牛血清白蛋白和肿瘤坏死因子-α的浓度没有影响。此外，顺式尿刊酸对细菌清除没有不良影响；用生理盐水或顺式尿刊酸处理的乳腺中大肠杆菌的CFU相当。
在奶牛中，注入顺式尿刊酸的大肠杆菌感染乳腺的牛奶NAGase和LDH活性均低于注入生理盐水的乳腺，这表明顺式尿刊酸减少了乳腺炎相关的组织损伤。此外，这些数据表明，通过乳房内注入可以在牛奶中达到治疗浓度的顺式尿刊酸。
