Carpinelli Nathaly Ana, Halfen Jessica, Michelotti Tainara Cristina, Rosa Fernanda, Trevisi Erminio, Chapman Jim D, Sharman Evin S, Osorio Johan S
Department of Dairy and Food Sciences, South Dakota State University, Brookings, SD 57007, USA.
Nucleo de Pesquisa, Ensino e Extensao em Pecuaria, Universidade Federal de Pelotas, Pelotas 96010610, Rio Grande do Sul, Brazil.
Animals (Basel). 2023 Jan 15;13(2):301. doi: 10.3390/ani13020301.
This study evaluated the effects of feeding a commercial yeast culture on blood biomarkers and polymorphonuclear leukocyte (PMNL) gene expression in dairy cows during the transition period until 50 d postpartum. Forty Holstein dairy cows were used in a randomized complete block design from -30 to 50 d. At -30 d, cows were assigned to a basal diet plus 114 g/d of top-dressed ground corn (control; = 20) or 100 g/d of ground corn and 14 g/d of a yeast culture product (YC; = 20). Blood samples were collected at various time points from -30 to 30 DIM to evaluate blood biomarkers and PMNL gene expression related to inflammation, liver function, and immune response. Liver function biomarkers, gamma-glutamyl transferase (GGT) and albumin were greater and lower, respectively, in YC cows in comparison to control. However, these biomarkers remained within physiological levels, indicating an active inflammatory process. Genes in PMNL expression related to inflammation (, , ), anti-inflammation (), and cell membrane receptors () were upregulated in the YC group in comparison to control. These results suggest that YC could stimulate a more active inflammatory response with signs of a resolution of inflammation in transition cows.
本研究评估了在围产期直至产后50天给奶牛饲喂商业酵母培养物对血液生物标志物和多形核白细胞（PMNL）基因表达的影响。40头荷斯坦奶牛采用随机完全区组设计，试验期从产前30天至产后50天。在产前30天，奶牛被分配到基础日粮加114克/天的撒施玉米粉（对照组；n = 20）或100克/天的玉米粉加14克/天的酵母培养物产品（YC组；n = 20）。在产前30天至产后30天的不同时间点采集血样，以评估与炎症、肝功能和免疫反应相关的血液生物标志物和PMNL基因表达。与对照组相比，YC组奶牛的肝功能生物标志物γ-谷氨酰转移酶（GGT）更高，而白蛋白更低。然而，这些生物标志物仍处于生理水平，表明存在活跃的炎症过程。与对照组相比，YC组中与炎症相关的（IL-1β、IL-6、TNF-α）、抗炎相关的（IL-10）以及细胞膜受体（TLR4）的PMNL表达基因上调。这些结果表明，YC可刺激围产期奶牛产生更活跃的炎症反应，并伴有炎症消退的迹象。
