Vera-Otarola J, Barría M I, León U, Marsac D, Carvallo P, Soza A, López-Lastra M
Laboratorio de Virología Molecular, Centro de Investigaciones Médicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
J Viral Hepat. 2009 Sep;16(9):633-43. doi: 10.1111/j.1365-2893.2009.01112.x. Epub 2009 Feb 25.
Peripheral blood mononuclear cells (PBMCs) from 45 treatment naïve, HIV-negative, chronically hepatitis C virus (HCV)-infected patients were analyzed for the presence of HCV RNA. Viral RNA was detected in 73% of the studied patients. Single-strand conformation polymorphism assays and sequence analysis of the HCV 5'untranslated regions amplified from RNA recovered from both Plasma and PBMCs suggested virus compartmentalization in 57.6% of patients studied. In summary, our study presents evidence that HCV RNA can be found in PBMCs of treatment naïve chronically infected patients that are not immunocompromised or co-infected with the human immunodeficiency virus.
对45例未经治疗、HIV阴性、慢性丙型肝炎病毒（HCV）感染患者的外周血单个核细胞（PBMC）进行分析，以检测HCV RNA的存在情况。在73%的研究患者中检测到病毒RNA。对从血浆和PBMC中回收的RNA扩增的HCV 5'非翻译区进行单链构象多态性分析和序列分析，结果表明，在57.6%的研究患者中存在病毒区室化现象。总之，我们的研究表明，在未经治疗的慢性感染且未免疫受损或未合并人类免疫缺陷病毒感染的患者的PBMC中可发现HCV RNA。
