人血清与外周血单个核细胞中丙型肝炎病毒高变区1准种的差异

Differences in hypervariable region 1 quasispecies of hepatitis C virus between human serum and peripheral blood mononuclear cells.

作者信息

Fujii K, Hino K, Okazaki M, Okuda M, Kondoh S, Okita K

机构信息

First Department of Internal Medicine, Yamaguchi University, School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1996 Aug 23;225(3):771-6. doi: 10.1006/bbrc.1996.1249.

DOI:10.1006/bbrc.1996.1249

PMID:8780688

Abstract

The complexity of hypervariable region 1 (HVR1) quasispecies of hepatitis C virus (HCV) in serum and peripheral blood mononuclear cells (PBMCs) was assessed at several time points in 11 patients with chronic hepatitis C. Polymerase chain reaction and single-strand conformation polymorphism analysis revealed that four patients (36%) showed serial changes in the complexity of HVR1 quasispecies, which were identical in both serum and PBMCs; two patients (18%) showed both serial changes in quasispecies and differences in quasispecies between serum and PBMCs; and five patients (45%) showed neither serial changes in nor quasispecies differences between serum and PBMCs. These results demonstrate both the existence of different quasispecies between serum and PBMCs and the infection of PBMCs by HCV.

摘要

在11例慢性丙型肝炎患者的多个时间点，对血清和外周血单核细胞（PBMC）中丙型肝炎病毒（HCV）高变区1（HVR1）准种的复杂性进行了评估。聚合酶链反应和单链构象多态性分析显示，4例患者（36%）的HVR1准种复杂性出现了连续变化，血清和PBMC中的变化相同；2例患者（18%）的准种出现了连续变化，且血清和PBMC中的准种存在差异；5例患者（45%）的血清和PBMC中既没有准种的连续变化，也没有准种差异。这些结果证明了血清和PBMC中存在不同的准种，以及HCV对PBMC的感染。