通过克隆分析和溴脱氧尿苷标记揭示果蝇成体大脑中的细胞增殖。
Cell proliferation in the Drosophila adult brain revealed by clonal analysis and bromodeoxyuridine labelling.
作者信息
von Trotha Jakob W, Egger Boris, Brand Andrea H
机构信息
The Wellcome Trust/Cancer Research UK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.
出版信息
Neural Dev. 2009 Mar 2;4:9. doi: 10.1186/1749-8104-4-9.
BACKGROUND
The production of new neurons during adulthood and their subsequent integration into a mature central nervous system have been shown to occur in all vertebrate species examined to date. However, the situation in insects is less clear and, in particular, it has been reported that there is no proliferation in the Drosophila adult brain.
RESULTS
We report here, using clonal analysis and 5'-bromo-2'-deoxyuridine (BrdU) labelling, that cell proliferation does occur in the Drosophila adult brain. The majority of clones cluster on the ventrolateral side of the antennal lobes, as do the BrdU-positive cells. Of the BrdU-labelled cells, 86% express the glial gene reversed polarity (repo), and 14% are repo negative.
CONCLUSION
We have observed cell proliferation in the Drosophila adult brain. The dividing cells may be adult stem cells, generating glial and/or non-glial cell types.
背景
迄今为止，在所有已检测的脊椎动物物种中，成年期新神经元的产生及其随后整合到成熟中枢神经系统的过程均已得到证实。然而，昆虫的情况尚不清楚，特别是有报道称果蝇成体大脑中不存在细胞增殖现象。
结果
我们在此报告，通过克隆分析和5'-溴-2'-脱氧尿苷（BrdU）标记，发现果蝇成体大脑中确实存在细胞增殖。大多数克隆聚集在触角叶的腹外侧，BrdU阳性细胞也是如此。在BrdU标记的细胞中，86%表达胶质细胞基因反转极性（repo），14%为repo阴性。
结论
我们观察到果蝇成体大脑中存在细胞增殖。分裂细胞可能是成体干细胞，可产生胶质细胞和/或非胶质细胞类型。
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