Patat Dilara, Nisari Mehtap, Ulger Harun, Ertekin Tolga, Dagli Ertugrul, Cayan Dicle, Al Ozge, Guler Hatice, Sengul Goksemin Fatma, Tastan Mustafa
Ankara Medipol University, Faculty of Medicine, Department of Anatomy, Anafartalar Campus, Eti, Celal Bayar Blv. No: 88, 06570, Ankara, Turkey.
Erciyes University, Faculty of Medicine, Department of Anatomy, Yenidoğan Mahallesi Turhan Baytop Sokak No: 1, 38280, Kayseri, Turkey.
Toxicol Res (Camb). 2024 Dec 9;13(6):tfae207. doi: 10.1093/toxres/tfae207. eCollection 2024 Dec.
In this study, effects of vitaminB on embryonic development have been investigated by supplying vitaminB on a hypoxia-induced embryo culture. 9.5-day-old embryos from Wistar albino adult pregnant rats were used in our experimental set up.10 μM and 100 μM vitaminB were added to culture medium which is then exposed to in vitro hypoxia. Additionally, 11.5-day-old embryos and yolksacs were examined morphologically. Different vitaminB doses are compared within experimental groups. It was found that both control and experimental groups in 11.5-day-old embryos are at same developmental stage. It was also determined that oxygen deficiency influenced embryonic development and yolk sac vascularity in hypoxia group, are lagging behind in all experimental groups ( < 0.05). However, the development of vitaminB embryos were similar to control group under normoxic conditions ( > 0.05). It was also observed that development was compensated through supplement of vitaminB to hypoxia group ). It was indicated that the development in H + 100 μM vitB groups was quite close to control group. However, development of H + 10 μM vitB embryos were in parallel with hypoxic group. Furthermore, H + 100 μM vitB group showed higher embryonic development than H + 10 μM vitB group ( < 0.05).VitaminB treatment has been used to prevent intrauterine growth restriction which can be caused by many different pharmacological agents. However, nobody has investigated effects of vitaminB on hypoxia-induced early embryo growth retardation. In the light of our findings, administration of 100 μM vitaminB restores damage of embryonic development due to hypoxia and this application also increases embryonic vascularity and circulation. Thus, supplementation of vitaminB can be offered as a therapeutic approach towards cell death and diseases such as neurovascular and cardiovascular diseases and in the near future.
在本研究中，通过在缺氧诱导的胚胎培养中提供维生素B，研究了维生素B对胚胎发育的影响。我们的实验采用了来自Wistar白化成年孕鼠的9.5日龄胚胎。将10μM和100μM的维生素B添加到培养基中，然后使其暴露于体外缺氧环境。此外，对11.5日龄的胚胎和卵黄囊进行了形态学检查。在实验组内比较了不同剂量的维生素B。结果发现，11.5日龄胚胎的对照组和实验组处于相同的发育阶段。还确定缺氧组的缺氧影响了胚胎发育和卵黄囊血管形成，在所有实验组中均滞后（P<0.05）。然而，在常氧条件下，维生素B处理的胚胎发育与对照组相似（P>0.05）。还观察到，通过向缺氧组补充维生素B，发育得到了补偿。结果表明，H+100μM维生素B组的发育与对照组相当接近。然而，H+10μM维生素B组胚胎的发育与缺氧组平行。此外，H+100μM维生素B组的胚胎发育高于H+10μM维生素B组（P<0.05）。维生素B治疗已被用于预防由许多不同药物制剂引起的宫内生长受限。然而，没有人研究过维生素B对缺氧诱导的早期胚胎生长迟缓的影响。根据我们的研究结果，给予100μM维生素B可恢复缺氧对胚胎发育的损害，并且这种应用还可增加胚胎血管形成和循环。因此，补充维生素B可作为一种治疗方法用于应对细胞死亡以及神经血管和心血管疾病等疾病，并且在不久的将来也可应用。
