分娩期间人子宫肌层中分泌型白细胞蛋白酶抑制剂的改变。
Alteration of secretory leukocyte protease inhibitor in human myometrium during labor.
作者信息
Li Aimin, Lee Richard H, Felix Juan C, Minoo Parviz, Goodwin Thomas M
机构信息
Department of Obstetrics and Gynecology, Women's and Children's Hospital, Los Angeles County-University of Southern California Medical Center, Los Angeles, CA 90033, USA.
出版信息
Am J Obstet Gynecol. 2009 Mar;200(3):311.e1-311.e10. doi: 10.1016/j.ajog.2008.10.045.
OBJECTIVE
Secretory leukocyte protease inhibitor (SLPI) has been shown to have antimicrobial and antiinflammatory properties. The aim of this study was to verify its expression in human myometrium.
STUDY DESIGN
Myometrium was obtained at time of cesarean delivery with (n = 9) or without (n = 11) labor. Expression of SLPI was detected using real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. SLPI expression relative to nuclear factor-kappaB p65 subunit was compared between subjects. SLPI response to inflammatory mediators was studied in myometrial explants.
RESULTS
SLPI was predominantly localized in the nuclei of myocytes and colocalized with CD68(+) macrophages. The nuclear immunoreactivity of SLPI was increased after the onset of labor and was associated with increased nuclear translocation of nuclear factor-kappaB p65 subunit. Treatment with lipopolysaccharide, interleukin-1beta, or tumor necrosis factor-alpha increased SLPI messenger RNA and protein concentrations slightly in myometrium explants.
CONCLUSION
SLPI was expressed in human myometrium and increased after the onset of labor.
目的
分泌型白细胞蛋白酶抑制剂（SLPI）已被证明具有抗菌和抗炎特性。本研究的目的是验证其在人子宫肌层中的表达。
研究设计
在剖宫产时获取子宫肌层组织，其中有产程的（n = 9）和无产程的（n = 11）。使用实时聚合酶链反应、酶联免疫吸附测定和免疫组织化学检测SLPI的表达。比较受试者之间SLPI相对于核因子-κB p65亚基的表达。在子宫肌层外植体中研究SLPI对炎症介质的反应。
结果
SLPI主要定位于肌细胞核内，并与CD68(+)巨噬细胞共定位。产程开始后，SLPI的核免疫反应性增加，并与核因子-κB p65亚基的核转位增加相关。用脂多糖、白细胞介素-1β或肿瘤坏死因子-α处理后，子宫肌层外植体中SLPI信使核糖核酸和蛋白质浓度略有增加。
结论
SLPI在人子宫肌层中表达，且在产程开始后增加。
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