Kageyama Kazunori, Hanada Komaki, Suda Toshihiro
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho Hirosaki, Aomori 036-8562, Japan.
Regul Pept. 2009 Jun 5;155(1-3):131-8. doi: 10.1016/j.regpep.2009.02.012. Epub 2009 Feb 28.
Urocortins (Ucns) are members of the corticotropin-releasing factor (CRF) family of peptides. Ucns would have potent effects on the cardiovascular system via the CRF receptor type 2 (CRF(2) receptor) in the cardiovascular system. However, an endogenous role and regulation of each Ucn have not been determined in the system. In the present study, we extended observations on stress or hormone-induced changes in Ucn gene expression in the cardiovascular system. Human umbilical vein endothelial cells (HUVECs) express Ucn1, Ucn2, and Ucn3 mRNAs, but not CRF mRNA, and the receptor, CRF(2 alpha) receptor mRNA. Lipopolysaccharides decreased Ucn1 mRNA levels, while it increased Ucn2 and Ucn3 mRNA levels in HUVECs. Interleukin-1beta decreased Ucn1 and Ucn2 mRNA levels, while it increased Ucn3 mRNA levels in HUVECs. Forskolin increased Ucn1 mRNA levels, while it decreased Ucn2 and Ucn3 mRNA levels. Ucns1-3 mRNA levels are differentially regulated in HUVECs. Differential regulation of Ucns1-3 mRNA may suggest differential roles of those in HUVECs.
尿皮质素（Ucns）是促肾上腺皮质激素释放因子（CRF）肽家族的成员。Ucns可通过心血管系统中的2型CRF受体（CRF(2)受体）对心血管系统产生强效作用。然而，该系统中每种Ucn的内源性作用和调节尚未确定。在本研究中，我们扩展了对应激或激素诱导的心血管系统中Ucn基因表达变化的观察。人脐静脉内皮细胞（HUVECs）表达Ucn1、Ucn2和Ucn3的mRNA，但不表达CRF mRNA以及受体CRF(2α)受体mRNA。脂多糖降低了HUVECs中Ucn1 mRNA的水平，同时增加了Ucn2和Ucn3 mRNA的水平。白细胞介素-1β降低了HUVECs中Ucn1和Ucn2 mRNA的水平，同时增加了Ucn3 mRNA的水平。福斯可林增加了Ucn1 mRNA的水平，同时降低了Ucn2和Ucn3 mRNA的水平。HUVECs中Ucns1 - 3 mRNA的水平受到不同的调节。Ucns1 - 3 mRNA的差异调节可能表明它们在HUVECs中的作用不同。
