用于蛋白质双模式检测的、由图案化多孔硅模板化的凝胶垫微阵列。
Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins.
作者信息
Chen Ling, Chen Zeng-Tai, Wang Jing, Xiao Shou-Jun, Lu Zu-Hong, Gu Zhong-Ze, Kang Lin, Chen Jian, Wu Pei-Heng, Tang Yan-Chun, Liu Jian-Ning
机构信息
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
出版信息
Lab Chip. 2009 Mar 21;9(6):756-60. doi: 10.1039/b821265a. Epub 2009 Feb 16.
A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.
一项概念验证研究通过以下步骤证明了在多孔硅芯片上的新型凝胶垫微阵列的可行性
利用表面硅氢键（Si-H）引发甲基丙烯酸聚乙二醇酯（PEGMA）的原子转移自由基聚合反应（ATRP）；将凝胶膜逐步化学转化为NTA-Ni2+/组氨酸标签蛋白系统；以及进行基质辅助激光解吸/电离质谱分析（MALDI MS）和荧光检测。
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