Chen Ling, Chen Zeng-Tai, Wang Jing, Xiao Shou-Jun, Lu Zu-Hong, Gu Zhong-Ze, Kang Lin, Chen Jian, Wu Pei-Heng, Tang Yan-Chun, Liu Jian-Ning
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
Lab Chip. 2009 Mar 21;9(6):756-60. doi: 10.1039/b821265a. Epub 2009 Feb 16.
A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.
利用表面硅氢键（Si-H）引发甲基丙烯酸聚乙二醇酯（PEGMA）的原子转移自由基聚合反应（ATRP）；将凝胶膜逐步化学转化为NTA-Ni2+/组氨酸标签蛋白系统；以及进行基质辅助激光解吸/电离质谱分析（MALDI MS）和荧光检测。
