Liu Changchun, Qiu Xianbo, Ongagna Serge, Chen Dafeng, Chen Zongyuan, Abrams William R, Malamud Daniel, Corstjens Paul L A M, Bau Haim H
Department of Mechanical Engineering and Applied Mechanics, Philadelphia, PA 19104-6315, USA.
Lab Chip. 2009 Mar 21;9(6):768-76. doi: 10.1039/b814322f. Epub 2008 Dec 5.
An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.
设计、构建并测试了一种廉价的手持式即时检测一次性独立免疫分析盒，其由用于泵送的气囊、计量室、试剂储存室、混合器和侧向流动条组成。该分析以连续流动形式进行。使用上转换磷光体（UCP）报告颗粒促进检测。各种试剂的自动定时泵送由弹簧加载定时器驱动。通过检测唾液样本中的HIV抗体证明了该分析盒的实用性，并通过非传染性半抗原化DNA分析进行了进一步评估。该分析盒比试纸条具有几个优点，如样品预处理、试剂的集成储存以及减少操作员误差和培训的自动化操作。本文所述的分析盒和致动器可以很容易地扩展到即时检测体液和其他液体中其他疾病的生物标志物。该系统特别适用于资源匮乏的国家，这些国家资金和训练有素的人员短缺。
