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基于微流控纸的离子浓度极化样品浓缩及智能手机检测

Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection.

作者信息

Li Xue, Niu Yanan, Chen Yunyi, Wu Di, Yi Long, Qiu Xianbo

机构信息

Institute of Microfluidic Chip Development in Biomedical Engineering, State Key Laboratory of Organic-Inorganic Composites, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

出版信息

Micromachines (Basel). 2016 Nov 4;7(11):199. doi: 10.3390/mi7110199.

DOI:10.3390/mi7110199
PMID:30404372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6189865/
Abstract

A simple method for microfluidic paper-based sample concentration using ion concentration polarization (ICP) with smartphone detection is developed. The concise and low-cost microfluidic paper-based ICP analytical device, which consists of a black backing layer, a nitrocellulose membrane, and two absorbent pads, is fabricated with the simple lamination method which is widely used for lateral flow strips. Sample concentration on the nitrocellulose membrane is monitored in real time by a smartphone whose camera is used to collect the fluorescence images from the ICP device. A custom image processing algorithm running on the smartphone is used to track the concentrated sample and obtain its fluorescence signal intensity for quantitative analysis. Two different methods for Nafion coating are evaluated and their performances are compared. The characteristics of the ICP analytical device especially with intentionally adjusted physical properties are fully evaluated to optimize its performance as well as to extend its potential applications. Experimental results show that significant concentration enhancement with fluorescence dye sample is obtained with the developed ICP device when a fast depletion of fluorescent dye is observed. The platform based on the simply laminated ICP device with smartphone detection is desired for point-of-care testing in settings with poor resources.

摘要

开发了一种利用离子浓度极化（ICP）和智能手机检测进行基于微流控纸的样品浓缩的简单方法。采用广泛用于侧流试纸条的简单层压方法，制造了由黑色背衬层、硝酸纤维素膜和两个吸收垫组成的简洁且低成本的基于微流控纸的ICP分析装置。通过智能手机实时监测硝酸纤维素膜上的样品浓缩情况，该手机的摄像头用于收集来自ICP装置的荧光图像。在智能手机上运行的自定义图像处理算法用于跟踪浓缩样品并获取其荧光信号强度以进行定量分析。评估了两种不同的Nafion涂层方法并比较了它们的性能。充分评估了ICP分析装置的特性，特别是具有有意调整的物理性质，以优化其性能并扩展其潜在应用。实验结果表明，当观察到荧光染料快速耗尽时，使用所开发的ICP装置可显著提高荧光染料样品的浓缩效果。基于简单层压的ICP装置和智能手机检测的平台适用于资源匮乏环境下的即时检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/c4796bd5fc8f/micromachines-07-00199-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/dd3a27408c9d/micromachines-07-00199-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/a2458a17db1c/micromachines-07-00199-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/21f9a51282bd/micromachines-07-00199-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/f4e2fd7b51c7/micromachines-07-00199-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/62bfa1fa7311/micromachines-07-00199-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/50122379389c/micromachines-07-00199-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/9c5128eb2594/micromachines-07-00199-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/c4796bd5fc8f/micromachines-07-00199-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/dd3a27408c9d/micromachines-07-00199-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/a2458a17db1c/micromachines-07-00199-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/21f9a51282bd/micromachines-07-00199-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/f4e2fd7b51c7/micromachines-07-00199-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/62bfa1fa7311/micromachines-07-00199-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/50122379389c/micromachines-07-00199-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/9c5128eb2594/micromachines-07-00199-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a8d/6189865/c4796bd5fc8f/micromachines-07-00199-g008.jpg

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本文引用的文献

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