Schmalenbach Inga, Pillen Klaus
Barley Genetics Research Group, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany.
Theor Appl Genet. 2009 May;118(8):1411-27. doi: 10.1007/s00122-009-0991-8. Epub 2009 Mar 3.
A malting quality quantitative trait locus (QTL) study was conducted using a set of 39 wild barley introgression lines (hereafter abbreviated with S42ILs). Each S42IL harbors a single marker-defined chromosomal segment from the wild barley accession 'ISR 42-8' (Hordeum vulgare ssp. spontaneum) within the genetic background of the elite spring barley cultivar 'Scarlett' (Hordeum vulgare ssp. vulgare). The aim of the study was (1) to verify genetic effects previously identified in the advanced backcross population S42, (2) to detect new QTLs, and (3) to identify S42ILs exhibiting multiple QTL effects. For this, grain samples from field tests in three different environments were subjected to micro malting. Subsequently, a line x phenotype association study was performed with the S42ILs in order to localize putative QTL effects. A QTL was accepted if the trait value of a particular S42IL was significantly (P < 0.05) different from the recurrent parent as a control, either across all tested environments or in a particular environment. For eight malting quality traits, altogether 40 QTLs were localized, among which 35 QTLs (87.5%) were stable across all environments. Six QTLs (15.0%) revealed a trait improving wild barley effect. Out of 36 QTLs detected in a previous advanced backcross QTL study with the parent BC(2)DH population S42, 18 QTLs (50.0%) could be verified with the S42IL set. For the quality parameters alpha-amylase activity and Hartong 45 degrees C, all QTLs assessed in population S42 were verified by S42ILs. In addition, eight new QTL effects and 17 QTLs affecting two newly investigated traits were localized. Two QTL clusters harboring simultaneous effects on eight and six traits, respectively, were mapped to chromosomes 1H and 4H. In future, fine-mapping of these QTL regions will be conducted in order to shed further light on the genetic basis of the most interesting QTLs.
利用一组39个野生大麦渗入系（以下简称为S42ILs）进行了麦芽品质数量性状位点（QTL）研究。每个S42IL在优良春大麦品种“Scarlett”（Hordeum vulgare ssp. vulgare）的遗传背景中含有一个来自野生大麦种质“ISR 42-8”（Hordeum vulgare ssp. spontaneum）的单一标记定义的染色体片段。该研究的目的是：（1）验证先前在高代回交群体S42中鉴定出的遗传效应；（2）检测新的QTL；（3）鉴定表现出多个QTL效应的S42IL。为此，对来自三个不同环境的田间试验的谷物样品进行了微麦芽制备。随后，对S42IL进行了品系×表型关联研究，以定位假定的QTL效应。如果特定S42IL的性状值与作为对照的轮回亲本在所有测试环境中或在特定环境中显著不同（P<0.05），则接受一个QTL。对于八个麦芽品质性状，共定位了40个QTL，其中35个QTL（87.5%）在所有环境中都是稳定的。六个QTL（15.0%）显示出性状改良的野生大麦效应。在先前使用亲本BC(2)DH群体S42进行的高代回交QTL研究中检测到的36个QTL中，18个QTL（50.0%）可以用S42IL集进行验证。对于品质参数α-淀粉酶活性和45℃下的哈通值，S42IL验证了在群体S42中评估的所有QTL。此外，定位了八个新的QTL效应和17个影响两个新研究性状的QTL。分别对八个和六个性状具有同时效应的两个QTL簇被定位到1H和4H染色体上。未来，将对这些QTL区域进行精细定位，以便进一步揭示最有趣的QTL的遗传基础。
