Sun Qinghua, Bai Yaohui, Zhao Cui, Xiao Yana, Wen Donghui, Tang Xiaoyan
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, People's Republic of China.
Bioresour Technol. 2009 Nov;100(21):5030-6. doi: 10.1016/j.biortech.2009.05.044. Epub 2009 Jun 18.
A bacterial strain, BW003, which utilized quinoline as its sole C, N and energy source, was isolated and identified as Pseudomonas sp. BW003 degraded 192-911 mg/l quinoline within 3-8 h with removal rates ranging from 96% to 98%. The optimum conditions for the degradation were 30 degrees C and pH 8. In the process of biodegradation, at least 43% of quinoline was transformed into 2-hydroxyquinoline, then 0.69% of 2-hydroxyquinoline was transformed into 2,8-dihydroxyquinoline, and then, presumably, into 8-hydroxycoumarin. Meanwhile, at least 48% of the nitrogen in quinoline was directly transformed into ammonia-N. An extra carbon source enhanced the nitrogen transformation from ammonia-N. Further experiments showed that, besides cell synthesis, BW003 transformed less than 6% of ammonia-N into nitrate through heterotrophic nitrification. In addition, BW003 contained a large plasmid, which may be involved in quinoline metabolism. The study indicates that quinoline and its metabolic products can be eliminated from wastewater by controlling the C/N ratio using BW003 as the bioaugmentation inoculum.
分离出一种细菌菌株BW003，它以喹啉作为唯一的碳、氮和能量来源，经鉴定为假单胞菌属。BW003在3至8小时内可降解192 - 911毫克/升的喹啉，去除率在96%至98%之间。降解的最佳条件是30摄氏度和pH值8。在生物降解过程中，至少43%的喹啉转化为2 - 羟基喹啉，然后0.69%的2 - 羟基喹啉转化为2,8 - 二羟基喹啉，接着可能转化为8 - 羟基香豆素。同时，喹啉中至少48%的氮直接转化为氨氮。额外的碳源增强了氨氮的氮转化。进一步的实验表明，除了细胞合成外，BW003通过异养硝化作用将不到6%的氨氮转化为硝酸盐。此外，BW003含有一个大质粒，可能参与喹啉代谢。该研究表明，以BW003作为生物强化接种物，通过控制碳氮比可从废水中去除喹啉及其代谢产物。
