Aref Amirreza, Horvath Robert, McColl James, Ramsden Jeremy J
Cranfield University, Department of Materials, Bedfordshire MK43 0AL, United Kingdom.
J Biomed Opt. 2009 Jan-Feb;14(1):010501. doi: 10.1117/1.3065541.
Modulation of the coupling of light into a waveguide via a grating, together with a novel approach to analyzing the data, is used to investigate the attachment of human embryonal carcinoma stem cells to three substrata: silica-titania (representative of artificial implants); poly-lysine (a commonly used laboratory cell culture substrate); and mucin (the coating of the mucosae). By considering both in-coupling peak width and position, the secretion of microexudate by the cells, the formation of filopodia, and the overall change in their shape (spreading) can be distinguished. This cannot be achieved by the conventional microscopic imaging approach. Moreover, we obtain the kinetics of these processes with excellent time resolution.
通过光栅对光耦合到波导中的调制，以及一种分析数据的新方法，被用于研究人类胚胎癌细胞系对三种基质的附着情况：二氧化硅 - 二氧化钛（代表人工植入物）；聚赖氨酸（一种常用的实验室细胞培养基质）；以及粘蛋白（粘膜的涂层）。通过考虑入射耦合峰的宽度和位置，可以区分细胞分泌的微渗出物、丝状伪足的形成以及它们形状的整体变化（铺展）。这是传统的显微镜成像方法无法实现的。此外，我们以出色的时间分辨率获得了这些过程的动力学。
