Pavlova Ina, Weber Crystal Redden, Schwarz Richard A, Williams Michelle D, Gillenwater Ann M, Richards-Kortum Rebecca
The University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas 78712, USA.
J Biomed Opt. 2009 Jan-Feb;14(1):014009. doi: 10.1117/1.3065544.
A Monte Carlo model with site-specific input is used to predict depth-resolved fluorescence spectra from individual normal, inflammatory, and neoplastic oral sites. Our goal in developing this model is to provide a computational tool to study how the morphological characteristics of the tissue affect clinically measured spectra. Tissue samples from the measured sites are imaged using fluorescence confocal microscopy; autofluorescence patterns are measured as a function of depth and tissue sublayer for each individual site. These fluorescence distributions are used as input to the Monte Carlo model to generate predictions of fluorescence spectra, which are compared to clinically measured spectra on a site-by-site basis. A lower fluorescence intensity and longer peak emission wavelength observed in clinical spectra from dysplastic and cancerous sites are found to be associated with a decrease in measured fluorescence originating from the stroma or deeper fibrous regions, and an increase in the measured fraction of photons originating from the epithelium or superficial tissue layers. The simulation approach described here can be used to suggest an optical probe design that samples fluorescence at a depth that gives optimal separation in the spectral signal measured for benign, dysplastic, and cancerous oral mucosa.
使用具有特定部位输入的蒙特卡罗模型来预测来自个体正常、炎症和肿瘤性口腔部位的深度分辨荧光光谱。我们开发此模型的目标是提供一种计算工具，以研究组织的形态特征如何影响临床测量的光谱。使用荧光共聚焦显微镜对测量部位的组织样本进行成像；针对每个个体部位，测量自体荧光模式作为深度和组织亚层的函数。这些荧光分布用作蒙特卡罗模型的输入，以生成荧光光谱预测，并将其与逐个部位的临床测量光谱进行比较。发现在发育异常和癌性部位的临床光谱中观察到的较低荧光强度和较长峰值发射波长与源自基质或更深纤维区域的测量荧光减少以及源自上皮或浅表组织层的光子测量比例增加有关。这里描述的模拟方法可用于建议一种光学探头设计，该设计在能为良性、发育异常和癌性口腔黏膜测量的光谱信号提供最佳分离的深度处对荧光进行采样。
