Wood Michael F G, Ghosh Nirmalya, Moriyama Eduardo H, Wilson Brian C, Vitkin I Alex
Ontario Cancer Institute, Division of Biophysics and Bioimaging, University Health Network, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada.
J Biomed Opt. 2009 Jan-Feb;14(1):014029. doi: 10.1117/1.3065545.
We demonstrate the first in vivo use of a Mueller matrix decomposition method for polarization-based characterization of tissue. Collagenase is injected into a region of dermal tissue in a dorsal skin window chamber in a nude mouse to alter the structure of the extracellular matrix. Mueller matrices for polarized light transmitted through the window chamber in the collagenase-treated region, as well as a distal control region, are measured. From the measured matrices, the individual constituent polarization properties of the tissue are extracted through polar matrix decomposition. Large decreases in birefringence and depolarization are seen in the collagenase-treated region due to the destruction of collagen, showing the potential for this method to monitor the organization and structural anisotropy of tissue. This study represents the first in vivo demonstration of a Mueller matrix decomposition method for polarimetric tissue characterization.
我们展示了穆勒矩阵分解方法首次在体内用于基于偏振的组织表征。将胶原酶注射到裸鼠背部皮肤窗口室的真皮组织区域，以改变细胞外基质的结构。测量通过胶原酶处理区域以及远端对照区域的窗口室透射的偏振光的穆勒矩阵。从测量的矩阵中，通过偏振矩阵分解提取组织的各个组成偏振特性。由于胶原蛋白的破坏，在胶原酶处理区域观察到双折射和去极化的大幅降低，这表明该方法具有监测组织的组织和结构各向异性的潜力。本研究首次在体内证明了穆勒矩阵分解方法用于偏振组织表征。
