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一种新型无创设备产生的雾化表面活性剂可减轻大鼠急性肺损伤。

Aerosolised surfactant generated by a novel noninvasive apparatus reduced acute lung injury in rats.

作者信息

Sun Yu, Yang Rui, Zhong Ji-gen, Fang Feng, Jiang Jin-jin, Liu Ming-yao, Lu Jian

机构信息

Department of Pathophysiology, College of Basic Medical Sciences, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.

出版信息

Crit Care. 2009;13(2):R31. doi: 10.1186/cc7737. Epub 2009 Mar 4.

DOI:10.1186/cc7737
PMID:19257907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2689462/
Abstract

INTRODUCTION

Exogenous surfactant has been explored as a potential therapy for acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). In the present study, a nebuliser driven by oxygen lines found in the hospital was developed to deliver aerosolised porcine pulmonary surfactant (PPS). We hypothesised that aerosolised surfactant inhaled through spontaneous breathing may effectively reduce severe lung injury.

METHODS

Rats were intravenously injected with oleic acid (OA) to induce ALI and 30 minutes later they were divided into five groups: model (injury only), PPS aerosol (PPS-aer), saline aerosol (saline-aer), PPS instillation (PPS-inst), and saline instillation (Saline-Inst). Blood gases, lung histology, and protein and TNF-alpha concentrations in the bronchoalveolar lavage fluid (BALF) were examined.

RESULTS

The PPS aerosol particles were less than 2.0 mum in size as determined by a laser aerosol particle counter. Treatment of animals with a PPS aerosol significantly increased the phospholipid content in the BALF, improved lung function, reduced pulmonary oedema, decreased total protein and TNF-alpha concentrations in BALF, ameliorated lung injury and improved animal survival. These therapeutic effects are similar to those seen in the PPS-inst group.

CONCLUSIONS

This new method of PPS aerosolisation combines the therapeutic effects of a surfactant with partial oxygen inhalation under spontaneous breathing. It is an effective, simple and safe method of administering an exogenous surfactant.

摘要

引言

外源性表面活性剂已被探索作为急性肺损伤（ALI）和急性呼吸窘迫综合征（ARDS）的一种潜在治疗方法。在本研究中，开发了一种由医院内的氧气管线驱动的雾化器，用于输送雾化的猪肺表面活性剂（PPS）。我们假设通过自主呼吸吸入雾化表面活性剂可能有效减轻严重肺损伤。

方法

给大鼠静脉注射油酸（OA）以诱导ALI，30分钟后将它们分为五组：模型组（仅损伤）、PPS雾化组（PPS-aer）、盐水雾化组（盐水-aer）、PPS滴注组（PPS-inst）和盐水滴注组（盐水-Inst）。检测血气、肺组织学以及支气管肺泡灌洗液（BALF）中的蛋白质和肿瘤坏死因子-α浓度。

结果

通过激光气溶胶颗粒计数器测定，PPS雾化颗粒大小小于2.0微米。用PPS雾化治疗动物显著增加了BALF中的磷脂含量，改善了肺功能，减轻了肺水肿，降低了BALF中的总蛋白和肿瘤坏死因子-α浓度，减轻了肺损伤并提高了动物存活率。这些治疗效果与PPS滴注组所见相似。

结论

这种新的PPS雾化方法结合了表面活性剂的治疗效果以及自主呼吸下的部分氧气吸入。它是一种有效、简单且安全的给予外源性表面活性剂的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/73cc6b8a24ff/cc7737-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/f80dd2982a03/cc7737-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/220627dc48a2/cc7737-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/5eb6ac605c52/cc7737-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/47dacc98f80e/cc7737-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/73cc6b8a24ff/cc7737-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/f80dd2982a03/cc7737-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/220627dc48a2/cc7737-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/5eb6ac605c52/cc7737-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/47dacc98f80e/cc7737-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d350/2689462/73cc6b8a24ff/cc7737-5.jpg

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