Hu Zhong-Yang, Zhang Le, Yang Qi-Dong
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Jun;33(6):494-9.
To explore the effect of apoB polymorphism on plasma lipid levels and cerebral hemorrhage in (CH) Changsha Han Chinese.
One hundred thirty CH patients and 100 normal people were involved. C7673T polymorphism of apoprotein B was analyzed by PCR-restriction fragment length polymorphism (PCR-PFLP); and the triglyceride(TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL), and low density lipoprotein-cholesterol(LDL) levels were examined by oxidase method. The serum level of lipoprotein(a) was determined by immunology method.
(1) Allele T frequencies of apoB C7673T in CH patients and the control group were 0.108 and 0.040, respectively. Allele T frequencies of apoB C7673T in the CH patients were significantly higher than those in the control group (P< 0.01). (2) In the CH patients, the levels of TC and LDLjC of the T/C gene type were significantly higher than those of the C/C gene type, while the levels of HDLjC of the T/C gene type were significantly lower than those of the C/C gene type (P< 0.05).
ApoB C7673T polymorphism may be related to cerebral hemorrhage, and the changing blood lipid level may increase the susceptibility of CH.
探讨载脂蛋白B（apoB）基因多态性对长沙汉族人群血脂水平及脑出血（CH）的影响。
纳入130例脑出血患者和100例正常人。采用聚合酶链反应-限制性片段长度多态性（PCR-PFLP）技术分析载脂蛋白B基因C7673T多态性；采用氧化酶法检测甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL）和低密度脂蛋白胆固醇（LDL）水平。采用免疫学法测定血清脂蛋白（a）水平。
（1）脑出血患者和对照组apoB C7673T基因T等位基因频率分别为0.108和0.040。脑出血患者apoB C7673T基因T等位基因频率显著高于对照组（P<0.01）。（2）在脑出血患者中，T/C基因型的TC和LDL-C水平显著高于C/C基因型，而T/C基因型的HDL-C水平显著低于C/C基因型（P<0.05）。
apoB C7673T基因多态性可能与脑出血有关，血脂水平的变化可能增加脑出血的易感性。
