Bai Yin-lan, Xue Ying, Wang Li-mei, Fan Ai-lin, Zhang Wei, Kang Jian, He Jun-jie, Xu Zhi-kai
Department of Microbiology, Fourth Military Medical University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Mar;25(3):211-4.
To evaluate immunoprophylaxis of recombinant Mycobacterium vaccae secreted MPT64 of Mycobacterium tuberculosis.
BALB/c mice were immunized with recombinant Mycobacterium vaccae secreted MPT64 of Mycobacterium tuberculosis. ELISA was used to detect the anti-MPT64 antibody titers and subtype in immunized mice sera. Splenocytes of immunized mice were separated and used to detect IFN-gamma and IL-12 levels, splenocytic proliferation, counts of CD4(+) and CD8(+) T cell percentage, cytolytic T lymphocyte specific lysis. The bacteria numbers in vaccination animal's lung and spleen were counted by colony forming units (CFUs) on plate.
Recombinant Mycobacterium vaccae secreted MPT64 of Mycobacterium tuberculosis could induce high level of specific IgG antibodies, stronger T cell proliferation response, production of IFN-gamma and IL-12, CD4(+) and CD8(+) cell percentages and CTL effect in mice. And an efficacy protection against Mycobacterium tuberculosis was also observed in mice model.
Recombinant Mycobacterium vaccae secreted MPT64 could induce high level humoral and cell mediated immune responses in mice and could be used as a candidate of new vaccine against TB.
评估分泌结核分枝杆菌MPT64的重组母牛分枝杆菌的免疫预防作用。
用分泌结核分枝杆菌MPT64的重组母牛分枝杆菌免疫BALB/c小鼠。采用酶联免疫吸附测定法检测免疫小鼠血清中抗MPT64抗体滴度及亚型。分离免疫小鼠的脾细胞，检测干扰素-γ和白细胞介素-12水平、脾细胞增殖、CD4(+)和CD8(+)T细胞百分比计数、细胞毒性T淋巴细胞特异性裂解。通过平板上的菌落形成单位（CFU）计数接种动物肺和脾中的细菌数量。
分泌结核分枝杆菌MPT64的重组母牛分枝杆菌可诱导小鼠产生高水平的特异性IgG抗体、较强的T细胞增殖反应、干扰素-γ和白细胞介素-12的产生、CD4(+)和CD8(+)细胞百分比以及细胞毒性T淋巴细胞效应。在小鼠模型中还观察到对结核分枝杆菌的有效保护作用。
分泌MPT64的重组母牛分枝杆菌可诱导小鼠产生高水平的体液免疫和细胞免疫反应，可作为新型抗结核疫苗的候选物。
